Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract

  • Ye Lin
  • Li Qin
  • Cai Xiaojun
  • Deng Ping
Article

Summary

By using RT-PCR and immunohistochemistry, the expressions of transforming growth factorβ 2(TGF-β2) mRNA, proliferating cell nuclear antigen (PCNA) and fibronection (FN) protein in lens epithelial cells (LECs) of age-related nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested that TGF-β2, PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation of LECs, and nuclear cataract to the proliferation of LECs.

Key words

TGF-β2 PCNA FN LECs cataract cortex nuclear 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Long A C, Colitz C M, Bomser J A. Apoptotic and necrotic mechanisms of stress-induced human lens epithelial cell death. Exp Biol Med (Maywood), 2004, 229(10): 1072Google Scholar
  2. 2.
    Huang Y, Wei H. Expression of transforming growth factor-beta in cultured normal human lens epithelia cells. J Huazhong Univ Sci Technology Med Sci, 2004, 24(3): 289.Google Scholar
  3. 3.
    Wormstone I M, Tamiya S, Eldred J Aet al. Characterization of TGF-beta2 signaling and function in a human lens cell line. Exp Eye Res, 2004, 78(3): 705PubMedCrossRefGoogle Scholar
  4. 4.
    Ihanamake T, Pelliniemi L J, Vuorio E. Collagens and collagen-related matrix components in the human and mouse eye. Pro Retin Eye Res, 2004, 23(4): 403CrossRefGoogle Scholar
  5. 5.
    Park J C, Park B J, Suh Het al. Comparative study on motility of the cultured fetal and neonatal dermal fibroblasts in extracellular matrix. Yonsei Med J, 2001, 42 (6): 587PubMedGoogle Scholar
  6. 6.
    Zhang X H, Sun H M, Yuan J Q. Extracellular matrix production of lens epithelial cells. J Cataract Refract Surg, 2001, 27(8): 1303PubMedCrossRefGoogle Scholar
  7. 7.
    Oharazawa H, Iharaki N, Lin L Ret al. The effects of extracellular matrix on cell attachment, proliferation and migration in a human lens epithelial cell line. Exp Eye Res, 1999, 69(6): 603PubMedCrossRefGoogle Scholar
  8. 8.
    Hall A B, Thompson J R, Deane J Set al. LOCSIII versus the Oxford Clinical Cataract Classification and Grading System for the assessment of nuclear, cortical and posterior subcapsular cataract. Ophthalmic Epidemiol, 1997, 4(4): 179.PubMedCrossRefGoogle Scholar
  9. 9.
    Shen G F, Mao H S. Molecular Cloning: A Laboratory Manual. Second ed. Hangzhou: Zhejiang Science and Technology Publishing House, 1988, 117Google Scholar
  10. 10.
    Sue Menko A. Lens epithelial cell differentiation. Exp Eye Res, 2002, 75(5): 485PubMedCrossRefGoogle Scholar
  11. 11.
    Matsushima H, Mukai K, Yoshida Set al. Effects of calcium on human lens epithelial cellsin vitro. Jpn J Ophthalmol, 2004, 48(2): 97PubMedCrossRefGoogle Scholar
  12. 12.
    Churchill G C, Louis C F. Ca(2+) regulation in differentiating lens cells in culture. Exp Eye Res, 2002, 75(1): 77PubMedCrossRefGoogle Scholar
  13. 13.
    Joo C K, Lee E H, Kim J Cet al. Degeneration and transdifferentiation of human lens epithelial cells in nuclear and anterior polar cataracts. J Cataract Refract Surg, 1999, 25(2): 652PubMedCrossRefGoogle Scholar
  14. 14.
    Zhang X H, Li X R, Sun H M. Transforming growth factor-β induce apoptosis in lens epithelial cells. Chin Ophthal Res, 2000, 18: 114Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Ye Lin
    • 1
  • Li Qin
    • 1
  • Cai Xiaojun
    • 1
  • Deng Ping
    • 1
  1. 1.Department of Ophthalmology, Zhongnan HospitalWuhan UniversityWuhanChina

Personalised recommendations