Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract
- 40 Downloads
By using RT-PCR and immunohistochemistry, the expressions of transforming growth factorβ 2(TGF-β2) mRNA, proliferating cell nuclear antigen (PCNA) and fibronection (FN) protein in lens epithelial cells (LECs) of age-related nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested that TGF-β2, PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation of LECs, and nuclear cataract to the proliferation of LECs.
Key wordsTGF-β2 PCNA FN LECs cataract cortex nuclear
Unable to display preview. Download preview PDF.
- 1.Long A C, Colitz C M, Bomser J A. Apoptotic and necrotic mechanisms of stress-induced human lens epithelial cell death. Exp Biol Med (Maywood), 2004, 229(10): 1072Google Scholar
- 2.Huang Y, Wei H. Expression of transforming growth factor-beta in cultured normal human lens epithelia cells. J Huazhong Univ Sci Technology Med Sci, 2004, 24(3): 289.Google Scholar
- 9.Shen G F, Mao H S. Molecular Cloning: A Laboratory Manual. Second ed. Hangzhou: Zhejiang Science and Technology Publishing House, 1988, 117Google Scholar
- 14.Zhang X H, Li X R, Sun H M. Transforming growth factor-β induce apoptosis in lens epithelial cells. Chin Ophthal Res, 2000, 18: 114Google Scholar