Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin
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An ultrastructural study of endocrine cells was performed in the pancreas of rats treated with a single dose of streptozotocin on the day of birth. Most of the B cells present at birth were destroyed by streptozotocin but some survived and could again synthesize insulin after eliminating their altered fragments in phago-lysosome structures. New B cells were produced primarily by the formation of new islets from the small pancreatic ducts. A study of mitosis in colchicine treated animals showed that most B cells present on day 4 were formed by mitosis of undifferentiated cells. No significant division of preexisting differentiated B cells could be shown in streptozotocin treated rats.
B cell regeneration in this newborn rat model is thus explained primarily by budding of new islets from the ducts.
Key wordsB cells Streptozotocin Regeneration of endocrine cells Experimental diabetes Endocrine pancreas
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