Virchows Archiv B

, 39:173 | Cite as

Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin

  • M. C. Dutrillaux
  • B. Portha
  • C. Rozé
  • E. Hollande


An ultrastructural study of endocrine cells was performed in the pancreas of rats treated with a single dose of streptozotocin on the day of birth. Most of the B cells present at birth were destroyed by streptozotocin but some survived and could again synthesize insulin after eliminating their altered fragments in phago-lysosome structures. New B cells were produced primarily by the formation of new islets from the small pancreatic ducts. A study of mitosis in colchicine treated animals showed that most B cells present on day 4 were formed by mitosis of undifferentiated cells. No significant division of preexisting differentiated B cells could be shown in streptozotocin treated rats.

B cell regeneration in this newborn rat model is thus explained primarily by budding of new islets from the ducts.

Key words

B cells Streptozotocin Regeneration of endocrine cells Experimental diabetes Endocrine pancreas 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • M. C. Dutrillaux
    • 1
  • B. Portha
    • 2
  • C. Rozé
    • 1
  • E. Hollande
    • 3
  1. 1.Biologie et Physiologie des Cellules Digestives (U239 Inserm, Dir. F. Potet)Faculté de Médecine X BichatParisFrance
  2. 2.Physiologie du Développement, Laboratoire associé au CNRS 307 (Dir. L. Picon)Université Paris VIIParisFrance
  3. 3.Biologie CellulaireUniversité Paul SabatierToulouseFrance

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