Biologia Plantarum

, Volume 32, Issue 6, pp 460–473 | Cite as

An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

  • J. Matoušek
  • P. Dědič
  • M. J. Beneš
  • P. Kopáček
  • Věra Turková
  • Ludmila Trněná
Original Paper
Received:
Accepted:

Abstract

A polyspecific antiserum against protein extracted from PSTV-infected tomato leaves was prepared and the IgGs were separated by affinity chromatography on a beaded cellulose adsorbent with an immobilized “healthy” antigen. The antibody not adsorbed entered into a preferential reaction with the antigen from PSTV-infected leaves as estimated by an enzyme-linked immunosorbent assay. The immunochemical reactions did not significantly exceed the control background, if antigens from tomato leaves infected with potato viruses X, Y and M were analyzed. By immunoblot technique we revealed, however, that several antigens not detected in healthy leaves appeared in the leaves infected either with PSTV or with viruses X and M. An accumulation of a major antigen having a molecular mass of about 70 kDa was observed in viroid-infected leaves only, suggesting the specificity for viroid infection. The antigen was found not to be an alkaline endoproteinase - the pathogenesis-related protein P-69.

Some antigens with molecular masses approximately 38.0, 23.7 and 22 kDa, which occurred in PSTV-infected leaves and in healthy calluses, were not detectable in PSTV-infected calluses.

No reaction exceeding the control level was observed using enzyme-linked immunosorbent assay for antigens from silver nitrate-treated tomato leaves, although such leaves showed symptoms similar to that caused by viroids.

Keywords

Potato Virus Tomato Leave Signal Recognition Particle Preferential Reaction Potato Spindle Tuber Viroid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • J. Matoušek
    • 1
  • P. Dědič
    • 2
  • M. J. Beneš
    • 3
  • P. Kopáček
    • 4
  • Věra Turková
    • 5
  • Ludmila Trněná
    • 1
  1. 1.Institute of Plant Molecular BiologyCzechoslovak Academy of SciencesČeské BudějoviceCzechoslovakia
  2. 2.Institute of Potato Research and BreedingHavlíčk∪v BrodHavlíčk∪v Brod
  3. 3.Institute of Macromolecular ChemistryCSASPraha 6
  4. 4.Lab.Anal.Chem. Biological Ctr.CSASBranišovskáČeské Budějovice
  5. 5.institute of Experimental BotanyCSASPraha 6Czechoslovakia

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