Necrotizing activity of tumor necrosis factor: histopathological investigation using Meth A sarcoma and granulation tissue
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The action of tumor necrosis factor (TNF) was investigated histopathologically in mice using methylcholanthrene A (Meth A)-induced sarcomas and granulation tissue induced by autotransplantation of fragments of liver and spleen. Highly purified murine TNF caused hemorrhagic necrosis of both the tumors and the granulation tissue. Proliferation of tumor capillaries, demonstrated microangiographically, occurred 2 h after TNF administration and hyperemia of tumor vessels was obvious after 3–6 h. Hyperemia and capillary leakage were also observed in the granulation tissue 6 h after TNF injection and hemorrhage was noted in the epidermis after 12 h. These results strongly suggest that the in vivo necrotizing action of TNF is mainly related to capillary injury.
Key wordsTumor necrosis factor Granulation tissue Capillary
- Freudenberg N, Joh K, Westphal O, Mittermayer CH, Freudenberg MA, Galanos CH (1984) Hemorrhagic tumor necrosis following endotoxin administration. I. Communication: morphological investigation on endotoxin-induced necrosis of the Methylcholanthrene (Meth A) tumor in the mouse. Virchows Arch (Pathol Anat) 403:377–389CrossRefGoogle Scholar
- Haranaka K, Satomi N, Sakurai A (1986a) Tumor necrosis factor (TNF) versus krebs statica (KBS). In: Tohno S (ed) Immunochemotherapy of cancer. Hirosaki University School of Medicine, pp 67–78Google Scholar
- Hirano-Mano Y, Sato N, Sawasaki Y, Haranaka K, Satomi N, Nariuchi H, Goto T (1987) Inhibition of tumor-induced migration of bovine capillary endothelial cells by mouse and rabbit tumor necrosis factor. J Natl Cancer Inst 78:115–120Google Scholar
- Sato N, Goto T, Haranaka K, Satomi N, Nariuchi H, Mano-Hirano Y, Sawasaki Y (1981) Actions of tumor necrosis factor on cultured vascular endothelial cells: morphologic modulation, growth inhibition, and cytotoxicity J Natl Cancer Inst 76:1113–1121Google Scholar