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Virchows Archiv B

, 53:353 | Cite as

Necrotizing activity of tumor necrosis factor: histopathological investigation using Meth A sarcoma and granulation tissue

  • Toshiaki Kawai
  • Nobuko Satomi
  • Noboru Sato
  • Akiko Sakurai
  • Katsuyuki Haranaka
  • Tamotsu Goto
  • Minoru Suzuki
Article

Summary

The action of tumor necrosis factor (TNF) was investigated histopathologically in mice using methylcholanthrene A (Meth A)-induced sarcomas and granulation tissue induced by autotransplantation of fragments of liver and spleen. Highly purified murine TNF caused hemorrhagic necrosis of both the tumors and the granulation tissue. Proliferation of tumor capillaries, demonstrated microangiographically, occurred 2 h after TNF administration and hyperemia of tumor vessels was obvious after 3–6 h. Hyperemia and capillary leakage were also observed in the granulation tissue 6 h after TNF injection and hemorrhage was noted in the epidermis after 12 h. These results strongly suggest that the in vivo necrotizing action of TNF is mainly related to capillary injury.

Key words

Tumor necrosis factor Granulation tissue Capillary 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Toshiaki Kawai
    • 1
  • Nobuko Satomi
    • 3
  • Noboru Sato
    • 2
  • Akiko Sakurai
    • 3
  • Katsuyuki Haranaka
    • 3
  • Tamotsu Goto
    • 2
  • Minoru Suzuki
    • 1
  1. 1.Department of PathologyNational Defense Medical CollegeTokorozawa, SaitamaJapan
  2. 2.Department of BiochemistryNational Defense Medical CollegeTokorozawa, SaitamaJapan
  3. 3.Department of Internal Medicine, Institute of Medical ScienceUniversity of TokyoMinato-ku, TokyoJapan

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