Virchows Archiv B

, Volume 58, Issue 1, pp 405–410

DNA ploidy and proliferative activity of human pulmonary epithelium

  • Shinshichi Hamada
  • Kenji Namura
  • Setsuya Fujita
  • Ryoji Kushima
  • Takanori Hattori
Article

Summary

DNA ploidy and distribution has been determined in normal and abnormal bronchial, bronchiolar and alveolar epithelium from 22 patients, aged between 0 and 85 years, 9 of whom had received chemotherapy for malignant disease. The DNA ploidy was diploid in all the specimens examined. The S + G2/M fraction was significantly greater in diseased than normal bronchial trees. In the bronchial epithelium, mean values ± the standard deviation (SD) were 5.5 + 2.2% vs 1.1±0.6%, in bronchiolar epithelium 4.6 ± 1.6% vs 1.0 ± 0.9% and in alveolar epithelium 4.6 ± 1.6% vs 0.8 ± 0.5%. The highest S + G2/M value of 8.9% was obtained from inflamed bronchial epithelium. Polyploid cells up to the octaploid range occurred infrequently but their incidence was slightly increased to between 0.16% and 0.9% in diseased lungs and in patients who had received chemotherapeutic drugs. It was concluded that (1) non-cancerous pulmonary epithelium is diploid, that (2) pulmonary epithelium shows steady-state renewal at all ages and polyploid cells are rare under normal conditions and that (3) the S + G2/M fraction increases up to approximately 10% in reactive proliferative states.

Key words

Human lung Aging DNA ploidy Proliferation Polyploidy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe S, Makimura S, Itabashi K, Nagai T, Tsuneta Y, Kawakami Y (1985) Prognostic significance of nuclear DNA content in small cell carcinoma of the lung. Cancer 56:2025–2030PubMedCrossRefGoogle Scholar
  2. Asamura H, Nakajima T, Mukai K, Noguchi M, Shimosato Y (1989) DNA cytofluorometric and nuclear morphometric analyses of lung adenocarcinoma. Cancer 64:1657–1664PubMedCrossRefGoogle Scholar
  3. Blenkinsopp WK (1967) Proliferation of respiratory tract epithelium in the rat. Exp Cell Res 46:144–154PubMedCrossRefGoogle Scholar
  4. Bodegom PC van, Baak JPA, Stroet-van Galen C, Schipper NW, Wisse-Brekelmans ECM, Vanderschueren RGJRA, Wagenaar SSC (1989) The percentage of aneuploid cells is significantly correlated with survival in accurately staged patients with stage 1 resected squamous cell lung cancer and long-term follow up. Cancer 63:143–147PubMedCrossRefGoogle Scholar
  5. Bunn PA, Carney DN, Gazdar AF, Whang-Peng J, Matthews MJ (1983) Diagnostic and biological implications of flow cytometric DNA content analysis in lung cancer. Cancer Res 1983:5026–5032Google Scholar
  6. Calabresi P, Parks RE (1985) Chemotherapy of neoplastic diseases. In: Gilman AG, Goodman LS, Rall TW, Murad F (eds) The pharmacological basis of therapeutics, 7th edn. Macmillan, New York, pp 1240–1306Google Scholar
  7. Cibas ES, Melamed MR, Zaman MB, Kimmel M (1989) The effect of tumor size and tumor cell DNA content on the survival of patients with stage I adenocarcinoma of the lung. Cancer 63:1552–1556PubMedCrossRefGoogle Scholar
  8. Evans MJ, Cabrai LJ, Stephens RJ, Freeman G (1973) Renewal of alveolar epithelium in the rat following exposure to NO 2. Am J Pathol 70:175–198PubMedGoogle Scholar
  9. Evans MJ, Johnson LV, Stephens RJ, Freeman G (1976) Renewal of the terminal bronchiolar epithelium in the rat following exposure to NO2 or 03. Lab Invest 35:246–257PubMedGoogle Scholar
  10. Fox B, Risdon RA (1968) Carcinoma of the lung and diffuse interstitial pulmonary fibrosis. J Clin Pathol 21:486–491PubMedCrossRefGoogle Scholar
  11. Fraire AE, Greenberg SD (1973) Carcinoma and diffuse interstitial fibrosis of lung. Cancer 31:1078–1086PubMedCrossRefGoogle Scholar
  12. Fujimoto T (1985) Cytofluorometric analysis of cell polyploidy of human various organs with aging and in diseased conditions (in Japanese). J Kyoto Pref Univ Med 94:403–421Google Scholar
  13. Fujita S (1981) Genesis and progression of gastric cancer from an aspect of cell kinetics (in Japanese). Trans Soc Pathol Jpn 70:23–54Google Scholar
  14. Greisen O (1971) The bronchial epithelium. Nucleic acid content in morphologically normal, metaplastic and neoplastic bronchial mucosae. Acta Otolaryngol (Stockh) [Suppl] 276:1–110Google Scholar
  15. Haddad R, Massaro D (1968) Idiopathic diffuse interstitial pulmonary fibrosis (fibrosing alveolitis), atypical epithelial proliferation and lung cancer. Am J Med 45:211–219PubMedCrossRefGoogle Scholar
  16. Hamada S (1989) Analysis of nuclear DNA content of lung cancer as a prognostic factor (in Japanese). Lung Cancer 29:448Google Scholar
  17. Hamada S, Fujita S (1983) DAPI staining improved for quantitative cytofluorometry. Histochemistry 79:219–226PubMedCrossRefGoogle Scholar
  18. Hamada S, Namura K, Itoh R, Fujita S (1987) Characteristics of colorectal epithelia and adenomas as revealed by DNA cytofluorometry. Jpn J Cancer Res 78:826–832PubMedGoogle Scholar
  19. Hamada S, Itoh R, Fujita S (1988) DNA distribution pattern of the so-called severe dysplasias and small carcinomas of the colon and rectum and its possible significance in the tumor progression. Cancer 61:1555–1562PubMedCrossRefGoogle Scholar
  20. Jones AW (1970) Alveolar cell carcinoma occurring in idiopathic interstitial pulmonary fibrosis. Br J Dis Chest 64:78–84PubMedCrossRefGoogle Scholar
  21. Kauffman SL (1972) Alteration in cell proliferation in mouse lung following urethane exposure. Am J Pathol 68:317–326PubMedGoogle Scholar
  22. Kauffman SL, Burri PH, Weibel ER (1974) The postnatal growth of the rat lung. II. Autoradiography. Anat Rec 180:63–76CrossRefGoogle Scholar
  23. Kawai T, Yakumaru K, Suzuki M, Kageyama K (1987) Diffuse interstitial pulmonary fibrosis and lung cancer. Acta Pathol Jpn 37:11–19PubMedGoogle Scholar
  24. Mayer FC, Liebow AA (1965) Relationship of interstitial pneumonia honeycombing and atypical epithelial proliferation to cancer of the lung. Cancer 18:322–351CrossRefGoogle Scholar
  25. Nasiell M, Kato H, Auer G, Zetterberg A, Roger V, Karlen L (1978) Cytomorphological grading and feulgen DNA-analysis of metaplastic and neoplastic bronchial cells. Cancer 41:1511–1521PubMedCrossRefGoogle Scholar
  26. Shimizu H (1985) Pathological study of lung cancer associated with idiopathic interstitial pneumonia with special reference to relationship between the primary site of lung cancer and honeycombing (in Japanese). Nippon Kyobu Shikkan Gakkai Zasshi 23:873–881PubMedGoogle Scholar
  27. Spain DM (1957) The association of terminal bronchiolar carcinoma with chronic interstitial inflammation and fibrosis of the lung. Am Rev Tuberc Pulm Dis 76:559–567Google Scholar
  28. Ten Velde GPM, Schutte B, Vermeulen A, Volovics A, Reynders MMJ, Blijham GH (1988) Flow cytometric analysis of DNA ploidy level in paraffin-embedded tissue of non-small-cell lung cancer. Eur J Cancer Clin Oncol 24:455–460CrossRefGoogle Scholar
  29. Trindelli-Danesi D, Teodori L, Mauro F, Modini C, Botti C, Cicconetti F, Stipa S (1987) Prognostic significance of flow cytometry in lung cancer, a 5-year study. Cancer 60:844–851CrossRefGoogle Scholar
  30. Volm M, Drings P, Mattern J, Sonka J, Vogt-Moykopf I, Wayss K (1985) Prognostic significance of DNA patterns and resistance-predictive tests in non-small-cell lung carcinoma. Cancer 56:1396–1403PubMedCrossRefGoogle Scholar
  31. Volm M, Bak M, Hahn EW, Mattern J, Weber E (1988) DNA and S-phase distribution and incidence of metastasis in human primary lung carcinoma. Cytometry 9:183–188PubMedCrossRefGoogle Scholar
  32. Wells AB (1970) The kinetics of cell proliferation in the tracheobronchial epithelia of rats with and without chronic respiratory disease. Cell Tissue Kinet 3:185–206PubMedGoogle Scholar
  33. Wright N, Alison M (1984) The biology of epithelial cell population, vol 2. Clarendon Press, Oxford, pp 1068–1078Google Scholar
  34. Zimmerman PV, Bint MH, Hawson GAT, Parsons PG (1987) Ploidy as a prognostic determinant in surgically treated lung cancer. Lancet II: 530–533CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Shinshichi Hamada
    • 1
  • Kenji Namura
    • 2
  • Setsuya Fujita
    • 2
  • Ryoji Kushima
    • 1
  • Takanori Hattori
    • 1
  1. 1.Department of PathologyShiga University of Medical ScienceOhtsuJapan
  2. 2.Department of PathologyKyoto Prefectural University of MedicineKamigyo-ku, KyotoJapan

Personalised recommendations