Advertisement

Virchows Archiv B

, 56:165 | Cite as

Renal oncocytoma

I. Cytochrome c oxidase in normal and neoplastic renal tissue as detected by immunohistochemistry- a valuable aid to distinguish oncocytomas from renal cell carcinomas
  • Monika Ortmann
  • Mathias Vierbuchen
  • Gerald Koller
  • Robert Fischer
Article

Summary

Using a polyclonal antibody raised against bovine heart cytochrome c oxidase, the occurrence of this mitochondrial marker enzyme has been investigated in 63 kidney tumors (ten renal oncocytomas, 43 renal cell carcinomas and ten tubulopapillary adenomas) as well as in normal renal tissue by an immunoperoxidase method (PAP-technique). The differentiation between renal oncocytomas and mitochondria-rich carcinomas represents a problem of histopathology since these tumors have a different prognosis and require different patient managements. The strong immunoreactivity in renal oncocytomas contrasted with the much weaker reactivity in renal carcinomas and adenomas. Even mitochondria-rich (granular cell type) carcinomas exhibited only moderate staining intensity. Furthermore, single strongly stained oncocytes or small complexes were sometimes detected in normal renal tissue. The demonstration of marked differences in enzyme content between renal oncocytomas and granular cell carcinomas renders this method suitable for unequivocal distinction between these renal neoplasms. The antibody proved to be a valuable marker for detecting “true” oncocytic transformation in renal tumors and was useful in defining even single oncocytes or small oncocytic lesions.

Key words

Cytochrome c oxidase Immunohistochemistry Renal cell carcinoma Renal oncocytoma 

References

  1. Alanen KA, Ekfors TO, Lipasti JA, Nurmi MJ (1984) Renal oncocytoma: the incidence of 18 surgical and 12 autopsy cases. Histopathol 8:731–737CrossRefGoogle Scholar
  2. Balogh K, Sanford IR (1965) Histochemical and electron microscopic studies of eosinophilic granular cells (oncocytes) in tumors of the parotid gland. Lab Invest 11:310–320Google Scholar
  3. Barnes CA, Beckman EN (1983) Renal oncocytoma and its congeners. Am J Clin Pathol 79:312–318PubMedGoogle Scholar
  4. Bedetti CD (1985) Immunocytochemical demonstration of cytochrome c oxidase with an immunoperoxidase method. J Histochem Cytochem 33:446–452PubMedGoogle Scholar
  5. Burstone MS (1959) New histochemical techniques for the demonstration of tissue oxidase (cytochrome oxidase). J Histochem Cytochem 7:112–122PubMedGoogle Scholar
  6. Choi H, Almagro UA, McManus JT, Norback DH, Jacobs SC (1983) Renal oncocytoma. A clinicopathologic study. Cancer 51:1887–1896PubMedCrossRefGoogle Scholar
  7. Eble JN, Hull MT (1984) Morphologic features or renal oncocytoma. Hum Pathol 15:1054–1061PubMedCrossRefGoogle Scholar
  8. Fairchild TN, Dail DH, Brannen GE (1983) Renal oncocytoma bilateral, multifocal. Urology 22:355–359PubMedCrossRefGoogle Scholar
  9. Fischer R (1961) Über den histochemischen Nachweis oxydativer Enzyme in Onkozyten verschiedener Ogane. Virchows Arch [Pathol Anat] 334:445–452CrossRefGoogle Scholar
  10. Graham RC, Lundholm U, Karnovsky MJ (1965) Cytochemical demonstration of peroxidase activity with 3-amino-9ethyl carbazole. J Histochem Cytochem 13:150–152PubMedGoogle Scholar
  11. Hackenbrock CR, Chan RM, Hammon K, Höckli L (1979) Localization of enzymes in mitochondrial membranes with ferritin conjugates of affinity purified antibodies. Methods Enzymol 56:683–707PubMedCrossRefGoogle Scholar
  12. Hamperl H (1962 a) Benign and malignant oncocytoma. Cancer 15:1019–1027PubMedCrossRefGoogle Scholar
  13. Hamperl H (1972 b) Onkozyten und Onkozytome. Virchows Arch [Pathol Anat] 335:452–483Google Scholar
  14. Klein MJ, Valensi QJ (1976) Proximal tubular adenomas of kidney with so-called oncocytic features. Cancer 38:906–914PubMedCrossRefGoogle Scholar
  15. Krizanac S, Vranesic D, Oberman B (1987) Oncocytomas of the kidney. Br J Urol 60:189–192PubMedCrossRefGoogle Scholar
  16. Lawrence WT, Bajallan NMS (1985) The importance of recognizing renal oncocytomas. Br J Urol 57:625–629PubMedGoogle Scholar
  17. Lewi HJE, Alexander CA, Fleming S (1986) Renal oncocytoma. Br J Urol 58:12–15PubMedGoogle Scholar
  18. Lieber MM, Tomera KM, Farrow GM (1981) Renal oncocytoma. J Urol 125:481–485PubMedGoogle Scholar
  19. Merino MJ, Livolsi VA (1982) Oncocytomas of the kidney. Cancer 50:1852–1856PubMedCrossRefGoogle Scholar
  20. Morales A, Wasan S, Bryniak S (1980) Renal oncocytomas: clinical, radiological and histological features. J Urol 123:261–264PubMedGoogle Scholar
  21. Rainwater LM, Farrow GM, Lieber MM (1986) Flow cytometry of renal oncocytoma: common occurrence of deoxyribonucleic acid polyploidy and aneuploidy. J Urol 135:1167–1171PubMedGoogle Scholar
  22. Rodrigues CA, Buskop A, Johnson J, Fromowitz F, Koss LG (1980) Renal oncocytoma. Acta Cytol (Baltimore) 24:355–359Google Scholar
  23. Sternberger LA (1979) Immunocytochemistry. John Wiley & Sons Inc, New YorkGoogle Scholar
  24. Talja MT, Kirisaari LM, Koivuniemi AP, Mäkinen JE, Lehtonen TA (1986) Diagnostic difficulties in oncocyte-containing renal carcinoma. Scand J Urol Nephrol 20:77–80PubMedCrossRefGoogle Scholar
  25. Thoenes W, Störkel S, Rumpelt HJ (1986a) Histopathology and classification of renal cell tumors (Adenomas, oncocytomas and carcinomas). The basic cytological and histopathological elements and their use for diagnostics. Pathol Res Pract 181:125–143PubMedGoogle Scholar
  26. Thoenes W, Störkel S, Rumpelt HJ, Jacobi GH (1986b) Das Nierenzellkarzinom eine Systematik auf Grund zytomorphologischer Merkmale. Zentralbl Allg Pathol Anat 132:503–513Google Scholar
  27. Tremblay G (1969) The oncocytes. Meth Achievm Exp Path 4:121–140Google Scholar
  28. Wenzel M, Alles JU, Fischer HP, Kracht J, Pascu F, Stambolis C (1986) Onkozytome der Niere. Fallberichte und Literaturübersicht. Pathologe 7:101–109PubMedGoogle Scholar
  29. Yu GMS, Rendler S, Herskowitz A, Molnar JJ (1980) Renal oncocytoma. Report of five cases and review of the literature. Cancer 45:1010–1018CrossRefGoogle Scholar
  30. Zerban H, Nogueira E, Riedasch G, Bannasch P (1987) Renal oncocytoma: origin from the collecting duct. Virchows Arch [Cell Pathol] 52:375–387CrossRefGoogle Scholar
  31. Zippel L (1942) Zur Kenntnis der Onkozyten. Virchows Arch [Pathol Anat] 308:360–382Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Monika Ortmann
    • 1
  • Mathias Vierbuchen
    • 1
  • Gerald Koller
    • 1
  • Robert Fischer
    • 1
  1. 1.Institute of PatholoyUniversity of CologneKöln 41Federal Republic of Germany

Personalised recommendations