Virchows Archiv B

, 52:33 | Cite as

In vitro labeling and tissue autoradiography of splenomegaly associated with portal hypertension

  • Kiyoshi Saitoh
  • Ryuichi Kamiyama
  • Shigeru Hatakeyama
  • Mitsuo Sugiura
Article
Received:
Accepted:

Summary

Sinus hyperplasia occurring in the congestive splenomegaly associated with portal hypertension is a interesting model of steady-state hyperplasia in an adult tissue. In vitro labeling of human splenic tissue with3H-thymidine and the demonstration of S-phase cells by autoradiography was performed to investigate the proliferative activity of sinuslining and cordai reticular cells in congestive splenomegaly compared with that in the normal spleen. The labeling index (%) of sinus-lining cells after 2 h incubation was 0.07±0.02 in the normal spleen and 0.26±0.03 in congestive splenomegaly (t = 4.553, P< 0.005, n = 11). This result indicated that sinus-lining cells have a long life span and that their proliferative activity is increased in congestive splenomegaly. On the other hand, the labeling of cordai reticular cells and arterial and venous endothelial cells was sparse or absent in both congestive splenomegaly controls, and these cells do not appear to be involved in sinus hyperplasia.

Key words

Splenomegaly Portal hypertension Organ culture Autoradiography 
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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Kiyoshi Saitoh
    • 1
  • Ryuichi Kamiyama
    • 1
  • Shigeru Hatakeyama
    • 1
  • Mitsuo Sugiura
    • 2
  1. 1.Department of Pathology, Faculty of MedicineTokyo Medical Dental UniversityJapan
  2. 2.Department of Surgery, Faculty of MedicineJuntendo UniversityJapan

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