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Journal of Tongji Medical University

, Volume 21, Issue 1, pp 32–34 | Cite as

Effect of antisense oligodeoxynucleotide directed to NF-κB-RelA on Bcl-xL mRNA in extended drug resistance leukemia cell line HL-60/E6

  • Cao Wenjing
  • Zhang Yaozhen
  • Zhang Donghua
  • Zou Ping
Article
  • 20 Downloads

Summary

To explore the effect of NF-κB on bcl-x gene transcription in extended drug resistance leukemia cell line HL-60/E6, drug-resistant subline HL-60/E6 was derived by intermittently exposing HL-60 cells to 6 ng/ml epirubicin. Indirect immunofluorescence was used to demonstrate the location of NF-κB-RelA in HL-60/E6 cells. FCM analysis and RT-PCR were used to detect the efficiency of liposome-mediated ODN transfection and the change of bcl-XL. mRNA levels after 5 μmol/L phosphorothioate (PS)-derivatized antisense (AS) oligodeoxynucleotide (ODN) directed to RelA was transferred into HL-60/E6 cells. The results showed that RelA remained persistently active and located at the nuclei of HL-60/E6 cells, but in the cytoplasm of HL-60 cells, the efficiency of liposomemediated ODN transfection was significantly higher than that of null ODN (P<0. 01 in 4 h, 6 h, 12 h, 24 h). Exposure of HL-60/E6 cells to 5 μmol/L AS-PS-ODN directed to RelA led to a maximal 40 % decline of bcl-xL mRNA levels within 8 h. The inhibition rate of bcl-XL. mRNA was (15±1. 79) %, (28±2. 34) %, (40±3.47) %, (20±1.54) % in 4 h,6h,8h, 15 h, respectively, but it was less than 15 % in control group. It was concluded that NF-κB was involved in regulating bcl-x transcription. It was suggested that NF-κB was an important factor for drug resistance in leukemia cells.

Key words

cell line HL-60/E6 RelA antisense oligodeoxynucleotide drug resistance bcl-XL 

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Copyright information

© Springer 2001

Authors and Affiliations

  • Cao Wenjing
    • 1
  • Zhang Yaozhen
    • 1
  • Zhang Donghua
    • 1
  • Zou Ping
    • 2
  1. 1.Department of Hematology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan
  2. 2.Institute of Hematology, Xiehe Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

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