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Journal of Tongji Medical University

, Volume 12, Issue 2, pp 107–110 | Cite as

Effect of chronic hypoxia on fibronectin in plasma, bronchoalveolar lavage fluid and liver of rats

  • Xu Yong-jian
  • Abe Tatsuya
  • Shiraishi Kouichiro
  • Motomiya Masakichi
Article
  • 17 Downloads

Summary

The present study was undertaken to measure fibronectin (FN) concentration in plasma and bronchoalveolar lavage fluid (BALF) with an enzyme linked immunosorbent assay (ELISA) method, to observe the distribution of FN in liver with an immunohistochemical stain method and to investigate the effect of chronic normobaric hypoxia on them in experimental rats. The results showed that chronic hypoxia caused a significant decrease in plasma FN concentration while BALF FN concentration and the distribution of FN in the liver were not significantly affected, suggesting that the change in FN in. the lower respiratory tract can not be reflected by the change in plasma FN concentration and that the decrease in plasma FN concentration can not be attributed to the change in liver FN secretion. The decrease in plasma FN concentration may lead to a decrease in the defence ability of the body and may contribute to the development of hypercoagulability observed in some patients with chronic hypoxia.

Key words

fibronectins hypoxia 

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References

  1. 1.
    Maunder RJ, et al. Measurement of plasma fibronectin in patients who develop the adult respiratory distress syndrome. J Lab Clin Med 1984;104:583PubMedGoogle Scholar
  2. 2.
    Yoder MC, et al. Plasma fibronectin in healthy newborn infants: respiratory distress syndrome and perinatal asphyxia. Pediatrics 1983;102:777CrossRefGoogle Scholar
  3. 3.
    Purves LR, et al. Fibronectin levels in normal subjects and patients admitted to a respiratory intensive care unit. S Afr Med J 1982;61:733PubMedGoogle Scholar
  4. 4.
    1988;11:15Google Scholar
  5. 5.
    1989;12:343Google Scholar
  6. 6.
    Kerr JS, et al. Reduction of chronic hypoxic pulmonary hypertension in the rat by an inhibitor of collagen production. Am Rev Respir Dis 1987;135:300PubMedGoogle Scholar
  7. 7.
    Nettelbladt O, et al. Hyaluronan (hyaluronic acid) in bronchoalveolar lavage fluid during the development of bleomycin induced alveolitis in the rat. Am Rev Respir Dis 1988;140:1028Google Scholar
  8. 8.
    Rennard SI, et al. Enzyme-linked immunoassay (ELISA) for connective tissue components. Analyt Biochem 1980; 104:205PubMedCrossRefGoogle Scholar
  9. 9.
    Bellay Y, et al. An immunocytochemical method for quantification of lung tissue fibronectin. Pro Soc Exp Bio Med 1988;188:159Google Scholar
  10. 10.
    Takusagawa K, et al. Immunoelectron microscopic observations on the localization of fibronectin in normal human lung. Tohoku J Exp Med 1986;150:209PubMedCrossRefGoogle Scholar
  11. 11.
    Hynes R, et al. Fibronectin: multifunctional modular glycoproteins. J Cell Biol 1982;95:369PubMedCrossRefGoogle Scholar
  12. 12.
    Oh E, et al. Deposition of plasma fibronectin in tissues. Proc Natl Acad Sci USA 1981;78:3128CrossRefGoogle Scholar
  13. 13.

Copyright information

© Springer 1992

Authors and Affiliations

  • Xu Yong-jian
    • 1
  • Abe Tatsuya
    • 2
  • Shiraishi Kouichiro
    • 2
  • Motomiya Masakichi
    • 2
  1. 1.Department of Internal Medicine, Tongji HospitalTongji Medical UniversityWuhan
  2. 2.Department of Internal Medicine, The Research Institute tor Tuberculosis and CancerTohoku UniversityJapan

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