Folia Microbiologica

, Volume 16, Issue 2, pp 110–113 | Cite as

Effect of Tween 80 on alkaloid-producing cultures ofClaviceps paspali

  • Z. Řeháček
  • S. C. Basappa


Addition of Tween 80 to submerged cultures ofClaviceps paspali (Stevens and Hall) growing in a simple defined medium increased biomass formation and caused a temporary change in alkaloid synthesis intensity. The Tween-supplemented culture reached maximal alkaloid yields four days earlier than the control. The shift of alkaloid production was associated with a shift of organic acids and amino acids in the cell-pool. Thus the maximal formation of alkaloids was characterized by a decrease in the amount of citric acid, by the disappearance of succinic, fumaric and oxaloacetic acids and by increased accumulation of methionine, cysteine, alanine and histidine. The slow alkaloid synthesis was accompanied by a relatively high content of citric and succinic acids in the cell-pool and by the absence of methionine. The data are consistent with the hypothesis that ergot alkaloids participate in the regulation of the metabolism of cultures.


Alkaloid Succinic Acid Fumaric Acid Submerged Culture Ergot Alkaloid 
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  1. Areamone, F., Chain, E. B., Ferretti, A., Minghetti, A., Pennella, P., Tonolo, A., Vero, L.:Production of a new lysergic acid derivative in submerged culture by a strain of Claviceps paspali Stevens & Hall. Proc. roy. Soc. (London) Ser. B 155: 26, 1961.Google Scholar
  2. Bu'Lock, J. D.:Intermediary metabolism and antibiotic synthesis. Adv. Appl. Microbiol. 3: 293, 1961.PubMedCrossRefGoogle Scholar
  3. Groeger, D., Tyler, V. E.:Alkaloid production by Claviceps paspali in submerged culture. Lloydia 16: 174, 1963.Google Scholar
  4. Kobel, H., Schreier, E., Rutschmann, J.:Ergot alkaloids. 60. 6-methyl delta 8,9-ergoline-8-carboxylic acid, a new ergoline derivative from cultures of Claviceps paspali. Helv. Chim. Acta 47: 1052, 1964.CrossRefGoogle Scholar
  5. Mizrahi, A., Miller, G.:Role of glycoles and tweens in the production of ergot alkaloids by Claviceps paspali. J. Bacteriol. 97: 1155, 1969.PubMedGoogle Scholar
  6. Partridge, S. M.:Filter paper partition chromatography of sugars. Biochem. J. 42: 238, 1948.PubMedGoogle Scholar
  7. Řeháček, Z., Kozová, J., Řičicová, A., Kašlík, J., Sajdl, P., Švare, S., Basappa, S. C.:Endogenous tryptophan role during submerged fermentation of ergot alkaloids. Fol. microbiol. 16: 35, 1971a.Google Scholar
  8. Řeháček, Z., Kozová, J., Sajdl, P., Malik, K. A.:The role of endogenous tryptophan and adenosine-5′-triphosphate in physiology of ergot alkaloid formation. (In: Vaněk, Z., Hoštálek, Z., Cudlín, J. (Eds.): Genetics of Industrial Microorganisms, 1971b.—In press.Google Scholar
  9. Roeder, K., Mutschler, E., Rochelmeyer, H.:Über die quantitative Bestimmung der Mutterkornalkaloide nach dünnschichtchromatographischer Trennung. Pharm. Acta Helv. 42: 407, 1967.Google Scholar
  10. Toennies, G., Kolb, T. T.:Technique and reagents for paper chromatography. Anal. Chem. 23: 823, 1951.CrossRefGoogle Scholar

Copyright information

© Academia, Publishing House of the Czechoslovak Academy of Sciences 1971

Authors and Affiliations

  • Z. Řeháček
    • 1
  • S. C. Basappa
    • 1
  1. 1.Department of Biogenesis of Natural Substances, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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