Immunohistochemical localization of gonadotropin-releasing hormone receptors (GnRHR) in the nervous system, Hatschek’s pit and gonads of amphioxus,Branchiostoma belcheri

  • Yongqiang Fang
  • Weiquan Huang
  • Lei Chen


Using gonadotropin-releasing hormone (GnRH) anti-idiotypic antibodies and APA immunohistochemical method, the immunoreactivity of GnRHR in the nervous system, Hatschek’s pit and gonads of amphioxus has been located. It is found for the first time that the immunoreactivity of GnRHR exists in the nerve cells and fibers in the amphioxus’s brain and nerve tube and the epithelial cells of Hatschek’s pit at the different stages of gonadal development. At the same time, it is also found that GnRHR also exists in the ovary and testis of different developed stages. These findings provide morphological new proof for the informative transfer and regulation between brain and Hatschek’s pit mediation by GnRHR, and for the understanding of the mechanism of action on the reproductive endocrine control axis among brain-Hatschek’s pit-gonads.


amphioxus GnRHR Immunohistochemistry nervous system gonads Hatschek’s pit 


  1. 1.
    Zhang, C. L., Yin, H., Identification of LH-RH in amphioxus,Acta Zool. Sinica (in Chinese), 1988, 34: 294.Google Scholar
  2. 2.
    Fang, Y. Q., Wang, H., Zhang, C. L., Correlation of annual change of luteinizing hormone-releasing hormone (LH-RH) with gonadal development in amphioxus,Science in China, Ser. B., 1991, 34: 814.PubMedGoogle Scholar
  3. 3.
    Fang, Y. Q., Zhao, W. X., Lin, Q. M., Hormonal regulation of oocyte development and maturation of amphioxus.Science in China, Ser. B, 1994, 37: 842.PubMedGoogle Scholar
  4. 4.
    Fang, Y. Q., Qi, X., Hong, G. Y., Study of gonadotropin-releasing hormone analogue induced spawning of amphioxus,J. Oceanography in Taiwan Strait, 1989, 8: 278.Google Scholar
  5. 5.
    Fang, Y. Q., Qi, X., Ultrastructural study of the Hatschek’ s pit epithelial cells of amphioxus,Science in China, Ser. B, 1989, 32: 1465.Google Scholar
  6. 6.
    Huang, W. Q., Zhang, C. L., Sun, L., Preparation and identification of GnRH anti-idiotypic antibody,J. Anatomica, 1994, 7C(suppl.): 353.Google Scholar
  7. 7.
    Kramanovie, L. Z., Stojilkovie, S. S., Mertz, L. al., Expression of gonadotropin-releasing hormone receptors and autocrine regulation of neuropeptide release in immortalized hypothalamic neurons,Proc. Natl. Acad. Sci. USA, 1993, 90: 3908.CrossRefGoogle Scholar
  8. 8.
    Reubi, J. C., Maurer, R., Visualization of LHRH receptors in the rat brain,Eur. J. Pharmacol., 1985, 106: 453.CrossRefGoogle Scholar
  9. 9.
    Habibi, H. R., Marchant, T. A., Nahormiak, C. al., Functional relationship between receptor binding and salmon gonadotropinreleasing hormone in the pituitary of goldfish (Carassius auratus),Biol. Reprod., 1989, 40: 1152.PubMedCrossRefGoogle Scholar
  10. 10.
    Weil, C., Crim, L. W., Wilson, C. al., Evidence of GnRH receptor in cultured pituitary cells of the winter flounder (Pseudopleuronectes americanus W), Gen. Comp. Endocrinol, 1992, 85: 156.PubMedCrossRefGoogle Scholar
  11. 11.
    Conn, P. M., Huckle, W. R., Duncan, J. al., The molecular mechanism of action of gonadotropin releasing hormone (GnRH) in the pituitary,Recent. Horm. Res., 1987, 43: 29.Google Scholar
  12. 12.
    Clayton, R. N., Harwood, J. P., Catt, K.J., Gonadotropin-releasing hormone analogus binds to luteal cells and inhibits progesterone production,Nature, 1979, 282: 90.PubMedCrossRefGoogle Scholar
  13. 13.
    Harwood, J. P., Clayton, R. N., Catt, K.J., Ovarian gonadotropin releasing hormone receptor, I. Properties and inhibition of luteal cell function,Endocrinology, 1980, 107: 407.PubMedCrossRefGoogle Scholar
  14. 14.
    Pati, D., Habibi, H. R., Gonadotropin-releasing hormone (GnRH) binding characteristics in the testis of goldfish (Carassius auratus)Exp. Zool., 1993, 267: 155.CrossRefGoogle Scholar
  15. 15.
    Pati, D., Habibi, H. R., Characterization of GnRH receptor in the goldfish ovary: variation during follicular development,Am. J. Physiol, 1993, 33: R227.Google Scholar
  16. 16.
    Zhang, Z. Y., Liu, Y. X., Zhu, H.H., The reproductive endocrinology of amphioxus, inFrontiers in Physiology Research (eds. Carlick, D. C., Roner, P. I.), Canberra: Australian Academy of Sciences, 1984, 79.Google Scholar
  17. 17.
    Fang, Y. Q., Position in the evolution of reproductive endocrine of amphioxus,Branchiostoma belcheri, Chinese Sci. Bulletin, 1998, 43(3): 177.CrossRefGoogle Scholar

Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Yongqiang Fang
    • 1
  • Weiquan Huang
    • 2
  • Lei Chen
    • 1
  1. 1.The Third Institute of OceanographyState Ocean AdministrationXiamenChina
  2. 2.The Fourth Military Medical CollegeXi’ anChina

Personalised recommendations