Effect of external pH on ethanol oxidation byCandida utilis
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Abstract
The external pH affects both ethanol and oxygen uptake rates by nongrowing cells ofCandida utilis suspended either in distilled water or in phthalate buffer. The buffering properties of organic acids control the maximum rates of exogenous respiration and ethanol uptake. The substrate limitation of ethanol uptake rate and endogenous respiration rate increase proportionally with increasing hydrogen ion concentration in the medium.
Keywords
Phthalate Phthalic Acid Oxygen Uptake Rate Ethanol Uptake Ethanol OxidationSymbols
- CE
concentration of ethanol, mmol/L
- [H+]
concentration of hydrogen ions in the medium, mol/L
- k1
parameter in Eq.4, mmol O2 per g dry matter per min
- k2
parameter in Eq.4, mmol O2·mol[H+]2 per g dry matter per h per L2
- KE
saturation constant for ethanol dissimilation, mmol/L
- pK
negative logarithm of dissociation constant, —log(mol/L)
- pH0
initial pH value of the medium, —log(mol/L)
- qE,qEmax
actual and maximum specific rate of ethanol dissimilation, mmol per g dry matter per h
- qO2,qO2
exogenous and endogenous respiration rate, mmol O2 per g dry matter per h
- t
time after ethanol feeding, h
- T
time constant, characterizing the duration of lag phase, h
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References
- Cartwright C.P., Juroszek J.G., Beavan M.G., Ruby F.M.S., De Morais S.M.F., Rose A.M.: Ethanol dissipates the protonmotive force across the plasma membrane ofSaccharomyces cerevisiae.J.Gen.Microbiol.132, 369–377 (1986).Google Scholar
- Casey G.P., Ingleder W.M.: Ethanol tolerance in yeasts.CRC Crit.Rev.Microbiol.27, 219–280 (1986).CrossRefGoogle Scholar
- Dombek K.M., Ingram L.O.: Magnesium limitation and its role in apparent toxicity of ethanol during yeast fermentation.Appl.Environ.Microbiol.52, 975–981 (1986a).PubMedGoogle Scholar
- Dombek K.M., Ingram L.O.: Nutrient limitation as a basis for apparent toxicity of low levels of ethanol during fermentation.J.Ind.Microbiol.1, 219–225 (1986b).CrossRefGoogle Scholar
- Dombek K.M., Ingram L.O.: Determination of intracellular concentration of ethanol inSaccharomyces cerevisiae during fermentation.Appl.Environ.Microbiol.51, 197–200 (1985).Google Scholar
- Guijarro J.M., Lagunas R.:Saccharomyces cerevisisae does not accumulate ethanol against a concentration gradient.J.Bacteriol.160, 874–878 (1984).PubMedGoogle Scholar
- Ingram L.O.: Effects of alcohols on microorganisms.Adv.Microbial Physiol.25, 254–300 (1984).Google Scholar
- Hoek J.B., Taraschi T.F.: Cellular adaptation to ethanol.Trends Biochem.Sci.13, 269–274 (1988).PubMedCrossRefGoogle Scholar
- Jimenez J., Benitez T.: Adaptation of yeast cell membranes to ethanol.Appl.Environ.Microbiol.53, 1196–1198 (1987).PubMedGoogle Scholar
- Jones R.P., Greenfield P.F.: Ethanol and the fluidity of the yeast plasma membrane.Yeast3, 223–232 (1987).PubMedCrossRefGoogle Scholar
- Jones R.P.: Factors influencing deactivation of yeast cells exposed to ethanol.J.Appl.Bacteriol.63, 153–164 (1987).PubMedGoogle Scholar
- Katchalsky A.: Polyelectrolytes.Pure Appl.Chem.26, 327–373 (1971).CrossRefGoogle Scholar
- Koukou A.I., Tsoukatos D., Drainas C.: Effect of ethanol on the phospholipid and fatty acid content ofSchizosaccharomyces pombe membranes.J.Gen.Microbiol.136, 1271–1277 (1990).PubMedGoogle Scholar
- Luong J.H.T.: Kinetics of ethanol inhibition in alcohol fermentation.Biotechnol.Bioeng.27, 280–285 (1985).CrossRefPubMedGoogle Scholar
- Martin-Rendon E., Jimenez J., Benitez T.: Ethanol inhibition ofSaccharomyces andCandida enzymes.Curr.Genet.15, 7–16 (1989).PubMedCrossRefGoogle Scholar
- Miller D.G., Groffiths-Smiths K., Algar E., Scopes R.K.: Activity and stability of glycolytic enzymes in the presence of ethanol.Biotechnol.Lett.4, 601–606 (1982).CrossRefGoogle Scholar
- Moulin G., Boze H., Galzy P.: Inhibition of alcoholic fermentation.Biotechnol.Bioeng.Rev.2, 365–382 (1984).Google Scholar
- Nabai R.C., Sa-Correia I., Viegas C.A., Novais J.M.: Influence of calcium ions on ethanol tolerance ofSaccharomyces baynaus and alcoholic fermentation by yeast.Appl.Environ.Microbiol.54, 2439–2446 (1988).Google Scholar
- Páca J., Grégr V.: Growth characteristics ofCandida utilis on volatile substrate in a multistage tower fermentor.Biotechnol.Bioeng.19, 539–554 (1977).PubMedCrossRefGoogle Scholar
- Páca J., Votruba J.: Effect of external pH on the respiration activity ofCandida utilis induced by ethanol.Appl.Microbiol.Biotechnol.33, 438–441 (1990).CrossRefGoogle Scholar
- Páca J., Votruba J.: Effect of external pH on acidification and excretion of ethanol intermediates byCandida utilis.Folia Microbiol.36, 485–492 (1991).Google Scholar
- Parsons R.V., McDuffie N.G., Din G.A.: pH inhibition of yeast ethanol fermentation in continuous culture.Biotechnol.Lett.6, 677–680 (1984).CrossRefGoogle Scholar
- Unger P., Voznakova Z., Páca J.: Analysis of cell metabolic products and fermentation gases by gas chromatography.J.Appl.Chem.Biotechnol.27, 150–154 (1977).CrossRefGoogle Scholar
- Votruba J., Parvez S., Sigler K.: Buffering capacity as an indicator of physiological state in continuous culture ofCandida utilis.Folia Microbiol.31, 312–318 (1986).CrossRefGoogle Scholar
- Zeuthen M.L., Dabrowa N., Aniebo C.M., Howard D.H.: Ethanol tolerance and the induction of stress proteins by ethanol inCandida albicans.J.Gen.Microbiol.134, 1375–1384 (1988).PubMedGoogle Scholar