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Biologia Plantarum

, 29:453 | Cite as

Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings

  • Marie Stiborová
  • Marta Ditrichová
  • Alena BŘEzinová
Article

Abstract

The effects of Cu2+, Zn2+, Cd2+ and Pb2+ on growth and the biochemical characteristics of photosynthesis were more expressed in barley (Hordeum vulgare L.) than in maize (Zea mays L.) seedlings. The barley and maize seedlings exhibited retardation in shoot and root growth after exposure of Cu2+, Cd2+ and Pb2+. The Zn2+ions practically did not influence these characteristics. The total protein content of barley and maize roots declined with an increase in heavy metal ion concentrations. The protein content of barley shoots was only slighly decreased with an increase in heavy metal ion concentrations, but the protein content in maize shoots was increased under the same conditions. The chlorophyll content was decreased in barley shoots and increased in maize. The ribulose-l,5-bisphosphate carboxylase (RuBPC, EC 4.1.1.39) and phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) activities were decreased drastically by Cu2+, Cd2+ and Pb2+ in thein vivo experiments. The tested heavy metal ions affect photosynthesis probably mainly by inhibition of these key carboxylating enzymes: this mechanism was studied in thein vitro experiments.

Keywords

Heavy Metal Malate Dehydrogenase Maize Seedling Maize Leave Bisphosphate Carboxylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. Baszynski, T.: Interference of Cd2+ in functioning of the photosynthetic apparatus of higher plants. - Acta Soc. Bot. Pol.55: 291–304, 1986.Google Scholar
  2. Bazzaz, M.B.,Govindjee: Effect of cadmium nitrate on spectral charecteristics and light reaction of chloroplates. -Environ. Lett.6: 1–12, 1974.PubMedCrossRefGoogle Scholar
  3. Bittel, J. E., Koeppe, D. E., Miller, R. J.: Sorption of heavy metal cations by corn mitochondria and the effects on electron and energy transfer reaction. - Physiol. Plant.30: 226–230, 1974.CrossRefGoogle Scholar
  4. Brändén, R, Nillsson, T., Styring, S.: Ribulose-1,5-bisphosphate carboxylase/oxygenase incubated with Cu2+ and studid by electron paramagnetic resonence spectroscopy. - Bio-chemistry23: 4373–4377, 1984a.Google Scholar
  5. Brändén, R, Nillsson, T., Styring, S.: An inytermediate formed by the Cu2+-activeted ribulose-1,5-bisphosphate carboxylase in the presence of ribulose-1,5-bisphosphate and O2. - Bio-chemistry23: 4378–4382, 1984b.Google Scholar
  6. Clijsters, H., van Assche, F.: Inhibitation of photosynthesis by metals. - Photosynth. Res.7: 31–40.1985.CrossRefGoogle Scholar
  7. Foy, C. D., Chaney, R. L., White, M. C.: The physiology of metal toxicity in plants. - Annu. Rev. Plant. Physiol.29: 511–566, 1978.CrossRefGoogle Scholar
  8. Hammond, P. B., Beliles, R. P.: Metals. - In: Doull, J., Klaassen, C.D., Amdur, M. O., (ed.): Toxicology. Pp. 409–467. McMillan, New York 1980.Google Scholar
  9. Iglecias, A. A., Andreo, C. S.: Involvement of thiol group in the activity of phosphoenolpyruvated carboxylase from maize leaves. - Photosynth. Res.5: 215–226, 1984.CrossRefGoogle Scholar
  10. Imai, I., Siegel, S.: A specefic response to toxic cadmium levels in red kidney bean embroy. - Physiol. Plant.29: 118–120. 1973.CrossRefGoogle Scholar
  11. Jastrow, J. D., Koeppe, D. E.: Uptake and effect of the cadmium in higher plants. - In: Unigan, J. O. (ed.): Cadmium in the environment. Part I. Pp. 608–637. Wiley Interscience, New York-Chichester 1980.Google Scholar
  12. Lagerwerft, J. V.: Lead, mercury and cadmium as environmental contaminants. - In: Mortvedt, J. J., Gioedano, P. M., Lindsay, W. L. (ed.): Micronutrients in Agriculture. Pp. 619 to 628. Soil Science Sooiety of America, Madison-Wisconsin 1972.Google Scholar
  13. Lamgreaux, R. J., Chaney, W. R.: The effect of cadmium on net photosynthesis, transpiration, and dark respiration of excised maple leaves. - Physiol. Plant.43: 231–236, 1978.CrossRefGoogle Scholar
  14. Leblova, S., Muocha, A., Stiborova, M.: [Plant metallothioneins.] In Csech. - Bull. ceskoslov. Spol. biochem.11: 62, 1983.Google Scholar
  15. Lorimer, G. H.: The carboxylation and oxygenation of ribulose 1,5-bisphosphate: the primary events in photosynthesis and photorespiration. - Anna. Rev. Plant Physiol.82: 349–388, 1981.CrossRefGoogle Scholar
  16. Lowby, O. H., Rosebrough, N. J., Farr, A. L., Randaill, A. J.: Protein measurements with the Folin phenol reagent. - J. biol. Chem.193: 266–275, 1951.Google Scholar
  17. Lucero, H. A., Andreo, C. S., Vallejos, R. H.: Sulphydryl groups in photosynthetic energy conservation. III. Inhibition of photophosphorylation in spinach chloroplasts by CdCl2. - Plant Sci. Lett.6: 309–313, 1976.CrossRefGoogle Scholar
  18. Nillsson, T., Brändén, R., Styring, S.: Distortion of the activator metal coordination during the turnover of cobalt-activated ribulosebisphosphate carboxylase/oxygenase. - Biochim. biophys. Acta788: 274–280, 1984.Google Scholar
  19. Rauser, W. E., Glover, J.: Cadmium-binding protein in roots of maize. - Can. J. Bot.62: 1845–1680, 1984.CrossRefGoogle Scholar
  20. Ryan, F. J., Tolbert, N. E.: Ribulose diphosphate carboxylase/oxygenase. 3. Isolation and properties. - J. biol. Chem.250: 4829–4233, 1975.Google Scholar
  21. Stiborová, M.: The role of cysteine and cystine residues in phosphoenolpyruvate carboxylase from inaize leaves. - Biochem. Physiol. Pflanzen 182: in press, 1987.Google Scholar
  22. Stiborová, M., Leblová, S.: The role of cystein SH group in the phtoephoenolpyruvate carbokylace molecule of maize. - Physiol, vèg.21: 936–942, 1983.Google Scholar
  23. Stiborová, M., Leblová, S., Zbrožek, J.: The subunit structure and the arnino acid composition of maize (Zea mays L.) phosphoenolpyruvate carboxylase.- Photosynthetica20: 173–180, 1986.Google Scholar
  24. Stobart, A. K., Griffiths, W. T., Ameen-Bukhari, I., Sherwood, R. P.: The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. - Physiol. Plant.63: 293–298, 1985.CrossRefGoogle Scholar
  25. Van Assche, F., Clijsters, H.: Multiple effects of heavy metal toxicity on photosynthesis. - In: Marcelle, R., Clijsters, H., Van Poucke, M. (ed.): Effects of Stress on Photosynthesis. Pp. 371–382. Nijhoff/Junk publ., The Hague 1983.Google Scholar
  26. Van Assche, F., Clusters, H.: Inhibition of photosynthesis inPhaseolus vulgaris by treatment with toxic concentration of zinc: effect on ribulose-l,5-bisphosphate carboxylase/oxygenase. - J. Plant Physiol.125: 355–360, 1986.Google Scholar
  27. Van Duijvendijk-Matteolli, M. A., Dbsmet, G. M.: On the inhibitory action of cadmium on the donor side of photosystem II in isolated chloroplasts.- Biochim. biophys. Acta408: 164–169, 1975.CrossRefGoogle Scholar
  28. Vernon, L. P.: Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. - Anal. Chem.32: 1144–1150, 1960.CrossRefGoogle Scholar
  29. Weigel, H. J.: Inhibition of photosynthetic reactions of isolated intact chloroplasts by cadmium. - J. Plant Physiol.119: 179–189, 1985.Google Scholar
  30. Woolhouse, H. W.: Toxicity and tolerance in the response of plants to metals. - In: Pirson, A., Zimmermann, M. H. (ed.): Encyclopedia of Plant Physiology. New Series 12C. Pp. 245 to 300. Springer-Verlag, Berlin - Heidelborg - Now York 1983.Google Scholar

Copyright information

© Academia 1987

Authors and Affiliations

  • Marie Stiborová
    • 1
  • Marta Ditrichová
    • 2
  • Alena BŘEzinová
    • 2
  1. 1.Department of BiochemistryCharles UniversityPraha 2
  2. 2.Institute of Experimental BotanyCzechoslovak Academy of SciencesPrahaCzechoslovakia

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