Science in China Series C: Life Sciences

, Volume 40, Issue 4, pp 379–383

Molecular cloning of GA-suppressed G2 pea genes by cDNA RDA

  • Yuxian Zhu
  • Yifeng Zhang
  • Huiying Li
Article

Abstract

GA-treated and non-treated G2 pea cDNAs were compared using a newly developed method called cDNA representational difference analysis (cDNA-RDA), and several GA-suppressed mRNAs were found. After cloning of the larger fragments PGAS1–3 (pea GA-suppressed cDNA 1–3), they were demonstrated to be expressed only in pea tissue not treated with GA3 through Northern analysis. Compared with subtractive hybridization and differential display techniques, this method not only can be easily manipulated but also has a relatively low rate of false positive and is highly repetitive. It is the major progress in molecular cloning techniques.

Keywords

G2 pea RDA molecular cloning GA-suppressed genes 

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Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Yuxian Zhu
    • 1
  • Yifeng Zhang
    • 1
  • Huiying Li
    • 1
  1. 1.State Key Laboratory of Protein Engineering and Plant Genetic EngineeringBeijingChina

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