Elimination of I131-labelled heterologous antigen from the blood of chickens after total body X-irradiation
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A study was made on the effect of ionizing radiation upon the rate of elimination of I131. labelled human serum albumin (HSA I131) from the blood and upon antibody formation in chickens irradited with 1,2 00R(i.e.with LD50) and injected with antigen 30 min, 6 days or 14 days after irradiation. The elimination curve from unirradiated control birds followed the typical three-phase pattern. The effect of irradiation was most marked with chickens injected with antigen 6 days after irradiation, resulting in an extension of the second phase with practically no third phase at all. Exposure to irradiation 30 min prior to antigen administration resulted in an extension of the second phase by 2 days as compared to the controls, with the onset of the third phase occurring on day 7. Irradiation 14 days prior to antigen administration resulted in an extension of the second phase by 1 day as compared to the controls, with the onset of the third phase occurring on day 6.
Elimination of HSA I131 in the second phase was more rapid than that of I131-labelled chicken serum albumin (CSA I131) no matter whether the chickens were irradiated or not. This suggests that the capacity of specific antigen uptake is not affected by irradiation.
Antigen elimination curves from control irradiated groups given CSA I131 followed the same pattern as that found in control unirradiated birds injected with homologous antigen.
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