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Biologia Plantarum

, Volume 27, Issue 4–5, pp 281–285 | Cite as

Hormonal factors stimulating starch accumulation in stem expiants of kale

  • J. Luštinec
  • K. Conrad
  • M. Kamínek
  • H. Keamell
  • G. Sembdner
Original Papers

Abstract

Hormonal factors stimulating starch accumulation were extracted with water from the vascular tissue containing expiants of kale (Brassica oleracea L. var.medullosa cv. Krasa) and fractioned by elution chromatography on a column of polyvinylpyrrolidone. Their activities were compared with those of some known phytohormones and other metabolites using a special bioassay based on protein synthesis-dependent starch accumulation in stem pith expiants free of vascular tissue. The highest relative activities of the hormonal factors compared were as follows: Most active eluate fraction (14.0), cytokinins (8.0), auxins (2.3), ethylene precursor (2.0), ABA (0.8), 24-epi-brassinolide (0.7), GA3 (0.4), water control (1.0).

Keywords

Vascular Tissue Zeatin Stimulate Activity Brassica Oleracea Starch Accumulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

IAA

indol-3-yl-acetic acid

2iP

N6-(Δ2-isoppent.enyl)adeuine

PVP

polyvinylpyrrolidone

SA

starch accumulation stimulating activity

Z

trans-zeatin

ZR

irans-zea,tinriboside

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Copyright information

© Academia 1985

Authors and Affiliations

  • J. Luštinec
    • 1
  • K. Conrad
    • 1
    • 2
  • M. Kamínek
    • 1
  • H. Keamell
    • 1
    • 3
  • G. Sembdner
    • 1
    • 3
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia
  2. 2.Section of BiologyErnst-Moritz-Amdt-UniversityGreifswaldG.D.R
  3. 3.Institute of Plant BiochemistryAcademy of SciencesHalle (S.)G.D.R.

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