Metal phthalocyanine as photosensitizer for photodynamic therapy (PDT)
- 307 Downloads
A series of sulfonated (S) phthalimidomethyl (P) zinc phthalocyanines (Pc) was synthesized in a reaction, in which both kinds of substituents were introduced to ZnPc simultaneously. The products were separated by HPLC. The five different fractions obtained were further purified by a membrane separation method, and then characterized by UV/Vis, IR, element analysis and the abilities to generate singlet oxygen upon irradiation by light as well as a preliminary determination ofin vitro antitumor activities. The results show that one of the five separating parts with formula of ZnPcS2P2 exhibited rather good PDT activity. The compound was further characterized by NMR, MS and thermal analysis. Studies onin vivo antitumor activities of ZnPcS2P2 as photosensitizer show that its inhibitory rate was up to 89.8% and 90.8% for S180 and U14 solid tumors transplanted in mice respectively when the dosage of drug was 2 mg/kg and the dosage of laser light with 670 nm wavelength was 72 J/cm2. Several structural factors influenced on the PDT activities were discussed.
Keywordsmetal phthalocyanine photodynamic therapy amphiphilicity anticancer photosensitizer
Unable to display preview. Download preview PDF.
- 2.Leznoff, C. C., Lever, A. B. P., Phthalocyanines: Properties and Applications, New York:VCH Publishers, 1989.Google Scholar
- 3.Hendersson, B. W., Dougherty, T. J., Photodynamic Therapy: Basic Principles and Clinical Applications, New York: Mar-cel Dekker Inc., 1992.Google Scholar
- 4.Huang Jian-Dong, Liu Er-Sheng, Huang Jin-Ling et al., The activities for anticancer and physicochimica properties of ZnPcSnPmseries, in Advences in Materials Research & Application (ed. China Materials Research Academy), Beijing: Chemical Industry Press, 1999, 740–742.Google Scholar
- 6.Liu Er-Sheng, Dai Zhi-Fei, Huang Jin-Ling et al., Syntheses of metal phthalocyanines and their photoinactivations on cancer cells, Acta Biochimica et Biophysica Sinica, 1998, 30(1): 31–34.Google Scholar
- 7.Liu Er-Sheng, Huang Jian-Dong, Chen Nai-Sheng et al., Studies on the synthesis and photodynamic antitumour activity of sulfonated phthalimidomethyl phthalocyanine zinc, Chinese Journal of Inorganic Chemistry, 1997, 13(4): 411–415.Google Scholar
- 8.Huang Jian-Dong, Liu Er-Sheng, Huang Jin-Ling et al., A study on the ability of some metal phthalocyanines for photo-generating singlet oxygen, Journal of Xiamen University (in Chinese), 1997, 36(3): 399–403.Google Scholar
- 9.Moser, J. G., Photodynamic Tumor Theropy, Amsterdam: Harwood Academic Pubisher, 1998.Google Scholar