Folia Microbiologica

, Volume 21, Issue 1, pp 10–20 | Cite as

Mapping of the chromosome ofMycobacterium phlei by means of mutagenesis of the replication point

  • M. Koníčková-Radochová
  • J. Koníček


The aim of the present work was to construct a replication map of the chromosome ofMycobacterium phlei. The method of mutagenesis of the replication point by means of nitrosoguanidine was applied to synchronously multiplying populations. Back mutations and forward mutations were induced m auxotrophic mutants PAmet and PAleu as well as in double auxotrophic mutants with methlomne as the reference marker and the following order of replication of eleven genes on the chromosome was thus established:leu-Eth, Res-Stm, Oyk-pur-met, arg, Cyk-Bac-inl


Cycloserine Nitrosoguanidine Ethionamide Nitroguanidine Replication Region 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1976

Authors and Affiliations

  • M. Koníčková-Radochová
    • 1
  • J. Koníček
    • 1
  1. 1.Department of Bacterial Genetics, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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