Folia Microbiologica

, 23:6 | Cite as

Production of amylase by a submerged culture ofAspergillus wentii

  • S. Sinha
  • S. L. Chakrabarty
Article

Abstract

Soluble starch was hydrolysed to maltose byAspergillus wentii Wehmer (IMI 17295). Studies on nutritional requirements ofAspergillus wentii for production of amylase revealed that the optimum conditions were achieved in fermentation culture medium containing 1% starch, and incubated at 20 °C for 3 days at pH 6.0. Tryptophan was the best nitrogen source. The amylase activity was completely inhibited when 1 mm sodium iodoacetate was incorporated into the medium. With 10 mm sodium citrate the amylase activity was increased from 3.51 to 6.0 mg/ml.

Keywords

Starch Amylase Nitrogen Source Maltose Submerged Culture 

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1978

Authors and Affiliations

  • S. Sinha
    • 1
  • S. L. Chakrabarty
    • 1
  1. 1.Department of MicrobiologyBose InstituteCalcuttaIndia

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