Folia Microbiologica

, Volume 29, Issue 1, pp 35–42 | Cite as

Improving the production of monensin byStreptomyces cinnamonensis

  • E. Královcová
  • V. Krumphanzl
  • Z. Vaněk


The production of monensin byStreptomyces cinnamonensis was increased by genetic improvement of the strain and by modification of cultivation conditions. The selection of a suitable strain and optimization of the fermantation process (temperature, aeration, addition of esters of oleic acid) resulted in a 30 fold increase of the monensin production.


Streptomyces Methyl Oleate Phenazine Submerged Culture Monensin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arai T.: Yumimycin, a novel antibiotic substance.Jap.Pat. 25 237 (1965).Google Scholar
  2. Burton K.: Determination of DNA concentration with diphenylamine, p. 163 in S. P. Colowick, N.O. Kaplan (Eds.):Methods in Enzymology, Vol. XIIB. Academic Press, New York 1968.Google Scholar
  3. Demain A.L.: Cellular and environmental factors affecting the synthesis and excretion of metabolitesJ.Appl.Chem.Biotechnol. 22, 345 (1972).CrossRefGoogle Scholar
  4. Falcao-Morais J.O., Goncalves-Lima O., Dalia-Maia M.H.:Streptomyces A20—producer of an antibiotic of the group of streptothricin—identified asStreptomyces cinnamonensis.Anais Soc.Biol.Pernambuco 14, 9 (1956):Chem.Abstr. 54, 14354a (1960).Google Scholar
  5. Haney M.E.Jr., Hoehn M.M.: Monensin, a new biologically active compound. I. Discovery and isolation.Antimicrob.Agents.Chemother. 349 (1967).Google Scholar
  6. Herold M., Hošťálek Z.: The carbohydrate metabolism of producing microorganism and the biosynthesis of tetracycline antibiotics, p. 93 in Z. Vaněk, Z. Hoštálek (Eds.):Biosynthesis of Antibiotics Substances Academic Press, New York 1965.Google Scholar
  7. Hesseltine C.W., Benedict R.G., Pridham F.G. Useful criteria for species differentiation in the genusStreptomyces.Ann.N.Y.Acad.Sci. 60, 136 (1954).PubMedCrossRefGoogle Scholar
  8. Jureček M.:Organic Analysis, p. 364. Publ. House Czech.Acad.Sci., Prague 1950.Google Scholar
  9. Mc Lamore W.M., Celmer W.D., Bogert V.V., Pennington F.C., Solomons I.A.: Structure and synthesis of thiazolidone antibiotic.J.Amer.Chem.Soc. 74, 2946 (1952).CrossRefGoogle Scholar
  10. Stapley E.O., Martinez M.J.: Antibiotic ensanchomycin.US Pat. 3 927 210 (1975).Google Scholar
  11. Stark W.M., Knox N.G., Westhead J.E.: Monensin, a new biologically active compound. II. Fermentation studies.Antimicrob. Agents Chemother. 353 (1967).Google Scholar
  12. Tax J., Sedmera P., Vokoun J., Urban J., Karnetová J., Stajner K., Vaněk Z., Krumphanzl V.: Phenazine derivatives fromStreptomyces cinnamonensis.Coll.Czech.Chem.Commun. 48, 527 (1983).Google Scholar
  13. Weinberg E.D.: Biosynthesis of secondary metabolites. Roles of trace metals.Adv.Microb. Physiol. 4, 1 (1970).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1984

Authors and Affiliations

  • E. Královcová
    • 1
  • V. Krumphanzl
    • 1
  • Z. Vaněk
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

Personalised recommendations