Application of chromogenic substrates to the determination of peptidases in mycobacteria
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Abstract
The problem of whether peptidase activity of mycobacteria is due to more specific enzymes or to a single, rather wide-spectrum, enzyme is approached by the application of definite chromogenic substrates aminoacyl naphthylamides, NA). This application might serve as an additional very important taxonomic tool for this bacterial family. The identification procedure described is simple and yields exact and rapid results. Other methods suggested for the determination of peptidase activity are presented. Lines of further research in the field are discussed.
Keywords
Peptidase Activity Chromogenic Substrate Diazonium Boron Trifluoride Diazonium Salt
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© Institute of Microbiology, Academy of Sciences of the Czech Republic 1967