Relationships between pathotypes and RAPDs, Gpi-allozyme patterns, mating types, and resistance to metalaxyl ofPhytophthora infestans in Canada in 1997

  • F. Daayf
  • H. W. (Bud) Platt
  • G. Mahuku
  • R. D. Peters
Article
Accepted:

Abstract

Relationships between pathotypes and other markers such as mating types, metalaxyl resistance, Gpi-allozyme and random amplified polymorphic DNA (RAPD) patterns, were investigated among 33 isolates ofPhytophthora infestans obtained from both potato and tomato across Canada in 1997. Pathogenicity testings, based on virulence against potato differentials carrying single-resistance genes and the size of lesions on leaves, revealed higher pathogenic diversity in 1997 than in the few past years, with more pathotypes and more race-complex isolates. A higher pathogenic diversity was observed within A2 (US-8) than within A1 (US-11) isolates. There was no correlation between the level of resistance to metalaxyl of an isolate and its pathogenicity. Nevertheless, when grouped into metalaxyl-sensitive (MS), -intermediate (MI), and -resistant (MR) classes, both average pathotype complexity and average lesion size were lowest for MS group. RAPD data revealed a good distinction between A1 (US-11) and A2 (US-8) mating type groups, and between isolates from British Columbia and those from other provinces. The detection in the last few years of both A1 and A2 mating types in Canada and the occurrence in 1997 of some isolates sharing different characteristics of both of these two groups suggest that sexual reproduction may have occurred locally.

Additional Key Words

Late blight of poato and tomato pathogen populations 

Resumen

Se investigaron las relaciones entre patotipos y otros mart-adores, como tipos de apareamiento, resistencia al metalaxil, alozima Gpi y patrones de polimorfismo en el ADN amplificado aleatoriamente (RAPD), entre 33 cultivos aislados dePhytophthora infestans obtenidos de cruces de papa y tomate en Canada en 1997. Las pruebas de patogenicidad, basadas en la virulencia contra diferenciales de papa portadoras de genes simples de resistencia y el tamaño de la lesión en las hojas, revelaron una mayor diversidad patógena en 1997 que en los años previos recientes, con aislamiento de mayor cantidad de patotipos y cepas complejas. En A2 (US-8) se observó una mayor diversidad patógena que en Al (US-11). No hubo correlación entre el nivel de resistencia a metalaxil de un cultivo aislado y su patogenicidad. No obstante, cuando se agrupó por clases, de acuerdo a sensibilidad al metalaxil (SM), -intermedia (MI) y -resistente (MR), tanto el promedio de complejidad del patotipo como el tamaño promedio de la lesión fueron menores para el grupo SM. Los datos del RAPD revelaron una gran diferencia entre los grupos de apareamiento A1 (US-11) y A2 (US-8) y entre los cultivos aislados en British Columbia con los de otras provincias. La detección en años recientes de los tipos de apareamiento A1 y A2 en Canadá y la ocurrencia en 1997 de algunos aislados que comparten características diferentes de ambos grupos, sugieren que la reproducción sexual podría haber ocurrido localmente.

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Literature Cited

  1. Burdon, J.J. and A.P. Roelfs. 1985. The effect of sexual and asexual reproduction on the isozyme structure of populations ofPuccinia graminis. Phytopathology 75:1068–1073.CrossRefGoogle Scholar
  2. Chycoski, C.I. and Z.K. Punja. 1996. Characteristics of populations ofPhytophthora infestans from potato in British Columbia and other regions of Canada during 1993 to 1995. Plant Dis 80:579–589.Google Scholar
  3. Daayf, F. and H.W. Platt. 1999. Assessment of mating types and resistance to metalaxyl of Canadian populations ofPhytophthora infestans in 1997. Am J Potato Res 76:287–295.Google Scholar
  4. Davidse, L.C., D. Looijen, L.J. Turkensteen, and D. Van Der Wal. 1981. Occurrence of metalaxyl-resistant strains ofPhytophthora infestans in Dutch potato fields. Neth J Plant Pathol 87:65–68.CrossRefGoogle Scholar
  5. Deahl, K.L., S.P. DeMuth, G. Pelter, and D.J. Ormrod. 1993a. First report of resistance ofPhytophthora infestons to metalaxyl in eastern Washington and southwestern British Columbia. Plant Dis 77:429.Google Scholar
  6. Deahl, K.L., S.P. DeMuth, S.L. Sinden, and A. Rivera-Peña 1995. Identification of mating types and metalaxyl resistance in North American populations ofPhytophthora infestans. Am Potato J 72:3549.CrossRefGoogle Scholar
  7. Deahl, K.L., R.W. Goth, R. Young, S.L. Sinden, and M.E. Gallegly. 1991. Occurrence of the A2 mating type ofPhytophthora infestans in the United States and Canada. Am Potato J 68:717–725.CrossRefGoogle Scholar
  8. Deahl, K.L. and D.A. Inglis. 1995. Occurrence of metalaxyl-insensitivePhytophthora infestans on Solanum sarachoides in north-western Washington. Plant Dis 79:540.Google Scholar
  9. Deahl, K.L., D.A. Inglis, and S.P. DeMuth. 1993b. Testing for resistance to metalaxyl inPhytophthora infestans isolates from northwestern Washington. Am Potato J 70:779–795.CrossRefGoogle Scholar
  10. Dorrance, A.E., D.A. Inglis, M.L. Dene, C.R. Brown, S.B. Goodwin, W.E. Fry, and K.L. Deahl. 1999. Characterization ofPhytophthora infestanspopulations in western Washington. Plant Dis 83:423–428.CrossRefGoogle Scholar
  11. Drenth, A., I.C.Q. Tas, and F. Govers. 1994. DNA fingerprinting uncovers a new sexually reproducing population ofPhytophthora infestans in The Netherlands. Eur J Plant Pathol 100:97–107.CrossRefGoogle Scholar
  12. Drenth, A., L.J. Turkensteen, and F. Govers. 1993. The occurrence of the A2 mating type ofPhytophthora infestans in The Netherlands; significance and consequences. Neth J Plant Pathol 99 (Suppl.3): 57–67.Google Scholar
  13. Fry, W.E., A. Drenth, L.J. Spielman, B.C. Mantel, L.C. Davidse, and S.B. Goodwin. 1991. Population genetic structure ofPhytophthora infestansin the Netherlands. Phytopathology 81:1330–1336.CrossRefGoogle Scholar
  14. Fry, W.E. and S.B. Goodwin. 1997a Re-emergence of potato and tomato late blight in the United States. Plant Dis 81:1349–1357.CrossRefGoogle Scholar
  15. Fry, W.E. and S.B. Goodwin. 1997b. Resurgence of the Irish potato famine fungus. BioScience 47:363–371.CrossRefGoogle Scholar
  16. Fry, W.E., S.B. Goodwin, A.T. Dyer, J.M. Matuszak, A. Drenth, P.W. Tooley, L.S. Sujkowski, Y.J. Koh, B.A. Cohen, L.J. Spielman, K.L. Deahl, D.A. Inglis, and K.P. Sandlan. 1993. Historical and recent migrations ofPhytophthora infestans: chronology, pathways, and implications. Plant Dis 77:653–661.Google Scholar
  17. Fry, W.E., S.B. Goodwin, J.M. Matuszak, L.J. Spielman, M.G. Milgroom, and A. Drenth. 1992. Population genetics and intercontinental migrations ofPhytophthora infestans. Annu Rev Phytopath 30:107–129.CrossRefGoogle Scholar
  18. Goodwin, S.B., B.A. Cohen, K.L. Deahl, and W.E. Fry. 1994a Migration from northern Mexico as the probable cause of recent genetic changes in populations ofPhytophthora infestans in the United States and Canada. Phytopathology 84:553–558.CrossRefGoogle Scholar
  19. Goodwin, S.B., B.A. Cohen, and W.E. Fry. 1994b. Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proc Nat Acad Sci USA 91:11591–11595.PubMedCrossRefGoogle Scholar
  20. Goodwin, S.B. and A. Drenth. 1997. Origin of the A2 mating type ofPhytophthora infestansoutside Mexico. Phytopathology 87:992–999.CrossRefPubMedGoogle Scholar
  21. Goodwin S.B., A. Drenth, and W.E. Fry. 1992b. Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs fromPhytophthora infestans. Curr Gen 22:107–115.CrossRefGoogle Scholar
  22. Goodwin, S.B., C.D. Smart, R.W. Sandrock, K.L. Deahl, Z.K. Punja, and W.E. Fry. 1998. Genetic change within populations ofPhytophthora infestansin the United States and Canada during 1994 to 1996: role of migration and recombination. Phytopathology 88:939–949.CrossRefPubMedGoogle Scholar
  23. Goodwin, S.B., L.J. Spielman, J.M. Matuszak, S.N. Bergeron, and W.E. Fry. 1992a Clonal diversity and genetic differentiation ofPhytophthora infestanspopulations in northern and central Mexico. Phytopathology 82:955–961.CrossRefGoogle Scholar
  24. Goodwin, S.B., L.S. Sujkowski, A.T. Dyer, B.A. Fry, and W.E. Fry. 1995a Direct detection of gene flow and probable sexual reproduction ofPhytophthora infestans in northern North America. Phytopathology 85:473–479.CrossRefGoogle Scholar
  25. Goodwin, S.B., L.S. Sujkowski, and W.E. Fry. 1995b. Rapid evolution of pathogenicity within clonal lineages of the potato late blight disease fungus. Phytopathology 85:669–676.CrossRefGoogle Scholar
  26. Goodwin, S.B., L.S. Sujkowski, and W.E. Fry. 1996a Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes ofPhytophthora infestans in the United States and Canada. Phytopathology 86:793–800.CrossRefGoogle Scholar
  27. Goodwin, S.B., L.S. Sujkowski, and W.E. Fry. 1996b. Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes ofPhytophthora infestans in the United States and Western Canada. Phytopathology 86:793–800.CrossRefGoogle Scholar
  28. Hohl, H.R. and K. Iselin. 1984. Strains ofPhytophthora infestans from Switzerland with A2 mating type behaviour. Trans Br Mycol Soc 83:529–530.CrossRefGoogle Scholar
  29. Kato, M., E.S. Mizubuti, S.B. Goodwin, and W.E. Fry. 1997. Sensitivity to protectant fungicides and pathogenic fitness of clonal lineages ofPhytophthora infestans in the United States. Phytopathology 87:973–978.CrossRefPubMedGoogle Scholar
  30. Koh, Y.J., S.B. Goodwin, A.T. Dyer, B.A. Cohen, A. Ogoshi, N. Sato, and W.E. Fry. 1994. Migrations and displacements ofPhytophthora infestanspopulations in east Asian countries. Phytopathology 84:922–927.CrossRefGoogle Scholar
  31. Lambert, D.H. and A.I. Currier. 1997. Differences in tuber rot development for North American clones ofPhytophthora infestans. Am Potato J 74:39–43.CrossRefGoogle Scholar
  32. Legard, D.E., T.Y. Lee, and W.E. Fry. 1995. Pathogenic specialization in Phytophthora infestans: aggressiveness on tomato. Phytopathology 85:1356–1361.CrossRefGoogle Scholar
  33. Levy, M., F.J. Correa-Victoria, R.S. Zeigler, S. Xu, and J.E. Hamer. 1993. Genetic diversity of the rice blast fungus in a disease nursery in Colombia. Phytopathology 83:1427–1433.CrossRefGoogle Scholar
  34. Levy, M., J. Romao, M.A. Marchetti, and J.E. Hamer. 1991. DNA fingerprinting with a dispersed repeated sequence resolves pathotype diversity in the rice blast fungus. Plant Cell 3:95–102.PubMedCrossRefGoogle Scholar
  35. Miller, J.S., P.B. Hamm, and D.A. Johnson. 1997. Characterization of thePhytophthora infestans populations in the Columbia basin of Oregon and Washington from 1992 to 1995. Phytopathology 87:656–660.CrossRefPubMedGoogle Scholar
  36. Nei, M. and W.-H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Nat Acad Sci USA 76:5269–5273.PubMedCrossRefGoogle Scholar
  37. Nelson, E.C. 1995. The cause of the calamity: the discovery of the potato blight in Ireland, 1845–1847, and the role of the National Botanic Gardens, Glasnevin, Dublin. In: Dowley, L.J., E. Bannon, L.R. Cooke, T. Keane, E. O’Sullivan (eds),Phytophthora infestans. Boole Press Ltd., Dublin, Ireland. pp. 1–11.Google Scholar
  38. Niederhauser, J.S. 1991.Phytophthora infestans: the Mexican connection. In: Lucas, J.A., R.C. Shattock, D.S. Shaw, L.R. Cooke (eds), Phytophthora. Cambridge University Press, Cambridge. pp. 25–45.Google Scholar
  39. Peters, R.D. 1998. Characterization of evolving populations ofPhytophthora infestanscausing late blight of potato in Canada. Ph.D. thesis. University of Guelph, Guelph, ON.Google Scholar
  40. Peters, R.D., H.W. Platt, and R. Hall. 1998a. Changes in structure of Canadian populations ofPhytophthora infestans based on specific virulence to selected potato clones. Potato Res 41:355–370.CrossRefGoogle Scholar
  41. Peters, R.D., H.W. Platt, and R. Hall. 1998b. Characterization of changes in populations ofPhytophthora infestans in Canada using mating type and metalaxyl sensitivity markers. Can J Plant Pathol 20:259–273.Google Scholar
  42. Rivera-Peña, A. 1990. Wild tuber-bearing species of Solanum and incidence ofPhytophthora infestans (Mont.) de Bary on the Western slopes of the Volcano Nevado de Toluca. 3. Physiological races of P. infestans. Potato Res 33:349–355.CrossRefGoogle Scholar
  43. Spielman, L.J., A. Drenth, L.C. Davidse, L.J. Sujkowski, W. Gu, P.W. Tooley, and W.E. Fry. 1991. A second world-wide migration and population displacement ofPhytophthora infestans? Plant Pathol 40:422–430.CrossRefGoogle Scholar
  44. Stevens, N.E. 1933. The dark ages in plant pathology in America: 1830–1870. J Wash Acad Sci 23:435–446.Google Scholar
  45. Sujkowski, L.S., S.B. Goodwin, A.T. Dyer, and W.E. Fry. 1994. Increased genotypic diversity via migration and possible occurrence of sexual reproduction ofPhytophthora infestans in Poland. Phytopathology 84:201–207.CrossRefGoogle Scholar
  46. Sujkowski, L.S., S.B. Goodwin, and W.E. Fry. 1996. Changes in specific virulence in polish populations ofPhytophthora infestans 1985–1991. Eur J Plant Pathol 102:555–561.CrossRefGoogle Scholar
  47. Swofford, D.L. and G.J. Olsen. 1990. Phylogeny reconstruction. In: Hills, D.M., and C. Moritz (eds), Molecular Systematics. Sinauer Associated Press, Sunderland, MA. pp. 411–501.Google Scholar
  48. Tooley, P.W., W.E. Fry, and M.J. Villarreal-Gonzalez. 1985. Isozyme characterization of sexual and asexualPhytophthora infestans populations. J Hered 76:431–435.Google Scholar
  49. Tooley, P.W., C.D. Therrien, and D.L. Riteh. 1989. Mating type, race composition, and isozyme analysis of peruvian isolates of Phytophthora infestans. Phytopathology 79:478–481.CrossRefGoogle Scholar
  50. Tooley, P.W., C.D. Themen, J.H. Sim, E. O’Sullivan, and L.J. Dowley. 1993. Mating type, nuclear DNA content, and isozyme genotypes of Irish isolates ofPhytophthora infestans. Mycol Res 97:1131–1134.Google Scholar
  51. Xia, J.Q., J.C. Correll, F.N. Lee, M.A. Marchetti, and D.D. Rhoads. 1993. DNA fingerprinting to examine microgeographic variation in the Magnaporthe grisea (Pyricularia grisea) population in two rice fields in Arkansas. Phytopathology 83:1029–1035.CrossRefGoogle Scholar

Copyright information

© Springer 2001

Authors and Affiliations

  • F. Daayf
    • 1
  • H. W. (Bud) Platt
    • 1
  • G. Mahuku
    • 1
  • R. D. Peters
    • 1
  1. 1.Agriculture and Agri-Food CanadaCrops and Livestock Research CentreCharlottetownCanada

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