Evaluation of adenosine deaminase activity and lipid peroxidation levels in diabetes mellitus

  • Anjali C. Warrier
  • Narasimha Y. Rao
  • Devdutta S. Kulpati
  • Tarun K. Mishra
  • Bhaskar C. Kabi
Diabetes Mellitus


There was increase in erythrocyte lipid peroxidation and adenosine deaminase (ADA) activity in red blood cells (RBC's), white blood cells (WBC's) and plasma, in 30 patients of confirmed diabetes mellitus, 10 each of insulin dependent diabetes mellitus (IDDM or type I), non-insulin dependent diabetes mellitus (NIDDM or type II) and diabetes with ketoacidosis when compared with 20 healthy individuals (controls). Glycosylated hemoglobin A1c%, plasma free fatty acid (FFA) and glycerol levels were also elevated in all the cases when compared with controls. The increase was more in diabetic ketoacidosis group. Significant positive correlations were seen between erythrocyte lipid peroxidation and ADA activities in RBC's, WBC's and plasma. It is suggested that decreased tissue adenosine levels due to increase in ADA activity, is related to the severity of hyperglycemia and lipid peroxidation in diabetes mellitus.

Key words

Adenosine deaminase Adenosine Lipid peroxidation Hyperglycemia Diabetes mellitus 


  1. 1.
    Heinz, F. (1984)Adenosine deaminase. In: Methods of enzymatic analysis, Ed. Bergmeyer H.U. Verlag Chemie. vol.IV, 3rd edn. p.308–315.Google Scholar
  2. 2.
    Reaven, G.M., Chang, H. and Hoffman, B.B. (1989)Horm. Metab. Res. 21, 168–171.PubMedGoogle Scholar
  3. 3.
    McLane, M.P., Black, P.R., Law, W.R. and Raymond, R.M. (1990)Diabetes 39, 123–129.CrossRefGoogle Scholar
  4. 4.
    Ismail, N.A. and Hems, D.A. (1978)Biochem. Pharmacol. 27, 1341–1345.PubMedCrossRefGoogle Scholar
  5. 5.
    Jain, S.K., Robert, M., Duett, J. and Herbs, J.J. (1989)Diabetes 38, 1539–1543.PubMedCrossRefGoogle Scholar
  6. 6.
    King, E.J. and Asatoor, A.M. (1975)In: Practical Clinical Biochemistry, Ed. Varley, H., Heinemann 4th edn. p. 80–96.Google Scholar
  7. 7.
    Fluckiger, R. and Winterhalter, K.H. (1976)FEBS. Lett. 71, 356–360.PubMedCrossRefGoogle Scholar
  8. 8.
    Gabbay, K.H., Sosenko, J.M., Banuchi, G.A., Minisohn, M.J. and Fluckiger, R. (1979)Diabetes, 28, 337–340.PubMedCrossRefGoogle Scholar
  9. 9.
    Nayak, S.S. and Pattabiraman, T.N. (1981)Clin. Chim. Acta. 169, 267–274.CrossRefGoogle Scholar
  10. 10.
    Dacie, J.V. and Lewis, S.M. (1984)In:Practical haematology: Basic haematological techniques, Churchill Livingston, Edinburgh, 6th ed. p 27–32.Google Scholar
  11. 11.
    Devic, M., Stankovic, H. and Boskovic, K. (1973)Clin. Chim. Acta. 45, 55–59.CrossRefGoogle Scholar
  12. 12.
    Korn, E.D. (1955)J. Biol. Chem. 215, 1–14.PubMedGoogle Scholar
  13. 13.
    Giusti, G. and Galanti, B. (1966)Soc. Ital. Biol. Sper. 42, 1316–1320.Google Scholar
  14. 14.
    Talwar, G.P. (1983)In:A handbook of Practical Immunology: Ed. Talwar, G.P. Vikas Publishers, New Delhi, p. 275–277.Google Scholar
  15. 15.
    Chaney, A.L. and Marbach, E.P. (1962)Clin. Chem. 8, 130–132.PubMedGoogle Scholar
  16. 16.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
  17. 17.
    Jamal, Z. and Saggerson, E.D. (1987)Biochem. J. 245, 881–886.PubMedGoogle Scholar
  18. 18.
    Klein, H.H., Ciarildi, T.P., Freidenberg, G.R. and Olefsky, J.M. (1987)Endocrinology 120, 2339–2345.PubMedCrossRefGoogle Scholar
  19. 19.
    Rutkiewicz, J. and Gorski, J. (1990)FEBS. Lett. 271, 79–80.PubMedCrossRefGoogle Scholar
  20. 20.
    Thliveris, J.A., Begleiter, A., Kobrinsky, N.L., Verburg, L., Dean, H.J. and Johnston, J.B. (1993)Biochem. Pharmacol. 46, 1071–1075.PubMedCrossRefGoogle Scholar
  21. 21.
    Newsholme, E.A., Blomstrand, E., Newell, J. and Pitcher, J. (1985)FEBS. Lett. 181, 189–192.PubMedCrossRefGoogle Scholar
  22. 22.
    Newsholme, E.A. (1992)Diabetes care 15, 1716–1720.PubMedCrossRefGoogle Scholar
  23. 23.
    Randle, P.J., Garland, P.B., Hales, C.N. and Newsholme, E.A. (1963)Lancet 1, 785–789.PubMedCrossRefGoogle Scholar
  24. 24.
    Tritsh, G.H., Paolini, N.S. and Bielat, K. (1985)Histochemistry 82, 281–285.CrossRefGoogle Scholar
  25. 25.
    Jain, S.K. (1984)J. Biol. Chem. 259, 3391–3394.PubMedGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 1995

Authors and Affiliations

  • Anjali C. Warrier
    • 1
    • 2
  • Narasimha Y. Rao
    • 1
    • 2
  • Devdutta S. Kulpati
    • 1
    • 2
  • Tarun K. Mishra
    • 1
    • 2
  • Bhaskar C. Kabi
    • 1
    • 2
  1. 1.Department of BiochemistryMaulana Azad Medical College and Associated Lok Nayak Jai Prakash Narayan HospitalNew Delhi
  2. 2.Department of MedicineMaulana Azad Medical College and Associated Lok Nayak Jai Prakash Narayan HospitalNew Delhi

Personalised recommendations