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Folia Microbiologica

, Volume 17, Issue 6, pp 517–521 | Cite as

Mechanism of resistance to 5-azacytidine inBacillus Subtilis

I. Isolation and some properties of mutants resistant to 5-azacytidine and 5-Aza-2′-deoxycytidine
  • V. Fučík
  • S. Zadražil
  • M. Jurovčík
  • Z. Šormová
Article

Abstract

Spontaneous mutants resistant to 5-azacytidine (5-AzCyd-r) and to 5-aza-2′-deoxycytidine (5-dAzCyd-r) were isolated inBacillus subtilis. There is no cross resistance between the two markers. Genetic transfer by a transformation process was possible with a low efficiency only with the 5-AzCyd-r marker. Independently isolated 5-AzCyd-r mutants did not show any differences in the level of resistance.

Keywords

Bacillus Subtilis Deoxycytidine Resistant Mutant Competent Culture Spontaneous Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chasin L. A., Magasanik B.: Gene expression after transformation inBacillus subtilis.J. Bacteriol.102, 661 (1970).PubMedGoogle Scholar
  2. Čihák A. Veselý J., Šorm F.: Incorporation of 5-azacytidine into liver ribonueleic aeids of leukemic mice sensitive and resistant to 5-azacytidine.Biochim. Biophys. Acta108, 516 (1965).PubMedGoogle Scholar
  3. Fučík V., Michaelis A., Rieger R.: On the induction of segment extension and chromatid structural changes inVicia faba chromosomes after treatment with 5-azacytidine and 5-azadeoxycytidine.Mutation Res.9, 599 (1970).PubMedGoogle Scholar
  4. Fučík V., Zadražil S., Šormová Z., Šorm F.: Mutagenic effects of 5-azacytidine in bacteria.Collection Czechoslov. Chem. Commun.30, 2883 (1965).Google Scholar
  5. Jurovčík M., Zadražil S., Fučík V., Šormová Z.: Mechanism of resistance to 5-azacytidine inBacillus subtilis. II. Utilization of 5-azacytidine and natural pyrimidines.Collection Czechoslov. Chem. Commun.37, 299 (1972).Google Scholar
  6. Marmur J.: A procedure for the isolation of deoxyribonucleic acid from microorganismsJ. Mol. Biol.3, 208 (1961).CrossRefGoogle Scholar
  7. Pískala S., Šorm F.: Nucleic acid components and their analogues. LI. Synthesis of 1-glycosyl derivatives of 5-azauracil and 5-azacytosineCollection Czechoslov. Chem. Commun.29, 2060 (1964).Google Scholar
  8. Piťhová P., Pískala A., Piťha J., Šorm F.: Nucleic acid components and their analogues. LXVI. Hydrolysis of 5-azacytidine and its connction with biological activity,Collection Czechoslov. Chem. Commun.30, 2801 (1965).Google Scholar
  9. Spizizen J.: Transformation of a biochemically deficient strain ofBacillus subtilis by deoxyribonucleate.Proc. Natl. Acad. Sci. U.S.44, 1072 (1958).CrossRefGoogle Scholar
  10. Veselý J., Čihák A., Šorm F.: Characteristics of mouse leukemic cells resistant to 5-azacytidine and 5-azacytidine and 5-aza-2′-deoxycytidine.Cancer Res.28, 1995 (1968).PubMedGoogle Scholar
  11. Young F. E., Spizizen J.: Physiological and genetic factors affecting transformation ofBacillus subtilis.J. Bacteriol.81, 823 (1961).PubMedGoogle Scholar
  12. Zadražil S., Fučík V., Jurovčík M., Šormová Z.: Mechanism of resistance to 5-azacytidine. III. Incorporation of 5-azacytidine into nucleic acids and its effect on the transforming activity of DNA.Collection Czechoslov. Chem. Commun.37, 309 (1972).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1972

Authors and Affiliations

  • V. Fučík
    • 1
  • S. Zadražil
    • 1
  • M. Jurovčík
    • 1
  • Z. Šormová
    • 1
  1. 1.Institute of Organic Chemistry and BiochemistryCzechoslovak Academy of SciencesPrague 6

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