Indian Journal of Clinical Biochemistry

, Volume 19, Issue 1, pp 71–75 | Cite as

Studies of biochemical parameters in breast cancer with and without metastasis



The study includes 102 confirmed cases of carcinoma breast with and without metastasis and 25 healthy non-pregnant females. They were evaluated for blood levels of Ferritin, GSH, LDH, ALP, GGT and Hb before and 21 days after mastectomy. A significant increase (p<0.001) was observed in ferritin, LDH and GSH levels in cancer patients without metastasis in comparison to normal control subjects. Patients with metastasis had further elevated (p<0.001) levels of Ferritin, ALP and GGT as compared to non-metastatic patients. Mastectomy in both the cases i.e. with and without metastasis resulted in non-significant decrease in all the biochemical parameters suggesting that longer follow up could confirm post surgery decrease in the biochemical parameters. The results of the study suggest cost effective, usefulness of Ferritin, ALP, GGT and GSH/Hb ratio in differentiating breast cancer patients with and without metastasis which can be assayed in smaller laboratories.

Key Words

Carcinoma breast Metastasis Glutathione Ferritin Lactate dehydrogenase Alkaline phosphatase Gamma glutamyl transpeptidase Diagnostic tools 


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  1. 1.
    Sharma, B.K. and Ray, A. (2000) Breast and Prostate Cancer. IJCB, 15, (Suppl.), 110–17.Google Scholar
  2. 2.
    Linblom, A. and Liljegreen, A. (2000) Tumor markers in malignancies. B.M.J., 320, 424–27.CrossRefGoogle Scholar
  3. 3.
    Burti’s, C.A. and Ashwood, E.R. (1994) Tietz textbook of clinical chemistry. Second edition, W.B. Saunders Company, Philadelphia pp. 814–15, 813, 848–49, 897–99.Google Scholar
  4. 4.
    Sadig, R.E.E. and Lai, L.C. (2000) Oestrogens and breast cancer. Clin. Biochemist. Rev. 21, 93–99Google Scholar
  5. 5.
    Beutler, E. (1971) Red cell metabolism: A manual of biochemical methods. Grune and Stration. New York, 103–5.Google Scholar
  6. 6.
    Worwood, M. (1986) Serum Ferritin. Clin. Sci. 70, 215–20.PubMedGoogle Scholar
  7. 7.
    Dacie, J.V. and Lewis, S.M. (1994) Practical haematology, Churchill Livingston, Edinburgh, 8th ed., pp. 49–82.Google Scholar
  8. 8.
    Guner, G., Kirkali, G., Yenisey, C., Tore, I.R. (1992) Cytosol and Serum ferritin in breast carcinoma. Cancer Lett. Dec. 24, 67 (2–3), 103–12.CrossRefGoogle Scholar
  9. 9.
    Perquin, M., Oster, T., Maul, A., Froment, N., Untereiner, M. and Bagrel, D. (2001) The Glutathione related detoxification system is increased in human breast cancer in correlation with clinical and histopathological features. J. Can. Res. Clin. Oncol. 127(6), 368–74.CrossRefGoogle Scholar
  10. 10.
    Aboughalia, A.H. and Fouad, I.M. (2000) Glutathione and its metabolizing enzymes in patients with different benign and malignant diseases. Clin. Biochem. 33 (8), 657–62.CrossRefGoogle Scholar
  11. 11.
    Kher, A., Maghe, G. and Deshpande, A. (1997) Significance of serum ferritin and lactate dehydrogenase in benign and malignant disease of breast. Indian J. Pathol. Microbiol. 40 (3), 321–6.PubMedGoogle Scholar
  12. 12.
    Albuquer que, K.V., Price, M.R., Badley, R.A., Jonrup, I., Pearson, D., Blamey, R.W. and Robertson, J.F. (1995) Pre-treatment serum levels of tumour markers in metastatic breast cancer: a prospective assessment of their role in predicting response to therapy and survival. Eur. J. Surg. Oncol. 21(5), 504–9.CrossRefGoogle Scholar
  13. 13.
    Letiagin, V.P. and Vysotskaia, I.V. (1995) Tumor marker CE A, CA 15-3, MCA, TPA, Ferritin and PTH in the diagnosis and monitoring of primary breast cancer. Vestin Ross Akad Med Nauk, 4, 10–14.Google Scholar
  14. 14.
    Aydiner, A., Topuz, E., Disci, R., Yasasever, V., Dincer, M., Dincol, K. and Bilge, N. (1994) Serum Tumor marker for detection of bone metastasis in breast cancer patients. Acta Oncol, 33 (2), 181–86.PubMedCrossRefGoogle Scholar
  15. 15.
    Kokocinska, D., Widala, E., Donocik, J. and Nolewajka, E. (1999) The value of evaluating tumor markers: CA15-3 and ferritin in blood serum of patients grouped as “high risk” for breast cancer. Przegl Lek. 56 (10), 664–67.PubMedGoogle Scholar
  16. 16.
    Elliott, R.L., Elliott, M.C., Wang, F. and Head, J.F. (1993) Breast carcinoma and the role of iron metabolism. A cytochemical tissue, culture, and ultrastructural study. Ann. N.Y. Acad. Sci. 698, 159–66.PubMedCrossRefGoogle Scholar
  17. 17.
    Devlin, T.M. (1997) Textbook of Biochemistry with clinical correlations. Fourth ed. Wiley-Liss. New York p. 484.Google Scholar
  18. 18.
    Sharabasy, M.M., Dosoky, I., Horia, H. and Khalaf, A.H. (1993) Elevation of glutathione, glutathione reductase and nucleic acids in both normal tissues and tumor of breast cancer patients. Cancer Lett. 72, 11–15.PubMedCrossRefGoogle Scholar
  19. 19.
    Murray, G.I., Burke, M.D. and Ewen, S.W.B. (1987) Glutathione localization in benign and malignant human breast lesions. Br. J. Cancer 55, 605–9.PubMedGoogle Scholar
  20. 20.
    Sieger, S., Bose, C.P., Younes, H., Thies, E., Hoppenkamp, S.R. and Younes, E. (1984) Glutathione and GSH dependent enzymes in the tumours and non-tumours mucosa of the human colon and rectum. J. Cancer Res. Clin. Oncol. 107, 288–340.Google Scholar
  21. 21.
    Beutler, E. and Gelbart (1985). Plasma glutathione in health and in patients with malignant disease. J. Lab. Clin. Med. 105, 581–84.PubMedGoogle Scholar
  22. 22.
    Kumaraguruparan, R., Subapriya, R., Kabalimoorthy, J. and Nagini, S. (2002) Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin. Biochem. 35(4), 275–79.PubMedCrossRefGoogle Scholar
  23. 23.
    Vaid, P. and Sharma, D.C. (1974) a clinico biochemical study of phosphohexose isomerase, allesterase and SH group. Ind. J. Can. Dec., 448–50.Google Scholar
  24. 24.
    Vanhoof, V.O., Vanoostrom, A.T., Lepoutre, L.G. and DeBroe, M.E. (1992) Alkaline phosphates isoenzyme patterns in malignancy disease. Clin. Chem. 38, 2546–2551.Google Scholar
  25. 25.
    Stieber, P., Mangal, D., Ritzke, C., Rossler, N., Kirsch, C.M. and Eiermann, W. (1992) Significance of bone alkaline phosphatase. CA15-3 and CEA in detection of bone metastasis during follow up of patients suffering from breast carcinoma. Eur. J. Clin. Chem. Clin. Biochem. 30, 809–14.PubMedGoogle Scholar
  26. 26.
    Ramaswamy, G., Rao, V.R., Krishnamoorthy, L., Ramesh, G., Gomathy, R. and Renukadevi, D. (2000) Serum levels of bone alkaline phosphatase in breast and prostate cancer with bone metastasis. Indian J. Clin. Biochem. 15 (2), 110–13.CrossRefGoogle Scholar
  27. 27.
    Lamerz, R., Stieber, P. and Fateh-Moghadam, A. (1993) Serum marker combinations in human breast cancer (review).In Vivo, 7 (613), 607–13.PubMedGoogle Scholar
  28. 28.
    Sharma, D.C., Mishra, S. and Sharma, P. (2001) Blood Glutathione level in breast cancer patients before and after surgery. J. Obst. Gyn. India. 51 (6), 150–51.Google Scholar

Copyright information

© Association of Clinical Biochemists of India 2004

Authors and Affiliations

  1. 1.Deptt. Of BiochemistrySMS Medical CollegeJaipur

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