Indian Journal of Clinical Biochemistry

, Volume 19, Issue 1, pp 14–20 | Cite as

Circadian periodicity of plasma lipid peroxides and anti-oxidant enzymes in pulmonary tuberculosis

  • Ranjana Singh
  • Rajesh K. Singh
  • Anil K. Tripathi
  • Nikhil Gupta
  • Ajai Kumar
  • Anil K. Singh
  • Abbas A. Mahdi
  • Rajendra Prasad
  • Raj K. Singh


The circadian periodicity of plasma lipid peroxide levels and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were studied in 50 clinically, bacteriologically and radiologically proven fresh cases of pulmonary tuberculosis (age: 21–45 years) and 60 age-matched healthy volunteers with diurnal activity from 06∶00 to about 22∶00 and nocturnal rest. A marked circadian variation in plasma lipid peroxide level was recorded in healthy subjects and pulmonary tuberculosis patients with significant amplitude and acrophase around 16∶21 and 17∶12 respectively. The acrophase tended to be delayed in tuberculosis patients. Furthermore, a statistically significant circadian rhythm was found in SOD, CAT and GPx activities in normal volunteers and pulmonary tuberculopsis patients. SOD and CAT enzyme activity was noted to be maximum at 06∶00 and minimum at 00∶00 in tuberculosis patients. The circadian acrophase for GPx activity was recorded at 16∶15 in normals and around 22∶45 in patients. Moreover, the activity was found to be decreased at all sampling hours during 24-hours sleep-awake period in patients in comparison to healthy counterparts. The MESOR and circadian amplitude also decreased markedly. The decreased activity of measured antioxidant enzymes in pulmonary tuberculosis patients could probably be associated with oxidative stress and/or decreased anti-oxidant defensive mechanism in such patients.


Tuberculosis Patient Pulmonary Tuberculosis Mean Square Pulmonary Tuberculosis Patient Circadian Periodicity 
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  1. 1.
    Plit, M.L., Theron, A.J., Fickl, H., Van Rensburg, C.E.J., Pendel, S. and Anderson, R. (1998) Influence of antimicrobial chemotherapy and smoking status on the plasma concentrations of vitamin C, vitamin E, b-Carotene, acute phase reactants, iron and lipid peroxide in patients with pulmonary tuberculosis. Intern. J. Tuberc. Lung Dis. 2: 590–596.Google Scholar
  2. 2.
    Halberg, F. (1969) Chronobiology. Ann. Rev. Physiol. 31: 675–725.CrossRefGoogle Scholar
  3. 3.
    Halberg, F., Halberg, E. and Carandente, F. (1976) Chronobiology and metabolism in the broader context of timely intervention and timed treatment. In: Diabetes Research Today, Meeting of the Minkowski Prize Winners, Symposia Medica Hoechest 12 (Capri), F.K. Schattaver Verlag, Stuttgart/New York, pp. 45–96.Google Scholar
  4. 4.
    Haus, E. and Halberg, F. (1980) Circadian time structure. In: Chronobiology—Principles and Applications to shifts in schedules, L.E. Schering and F. Halberg, (eds), Sijthoff and Noordhoff, Alphen aan den Rijn, The Netherlands, pp. 47–94.Google Scholar
  5. 5.
    Halberg, F. (1983) Quo vadis basic and clinical chronobiology: promise for health maintenance. Amer. J. Anat. 168: 543–594.PubMedCrossRefGoogle Scholar
  6. 6.
    Halberg, F., Cornelissen, G. and Bakken, E. (1990) Caregiving merged with chronobiologic outcome assessment, research and education in health maintenance organizations. Progress in Clinical and Biological Research 341B: 491–549.PubMedGoogle Scholar
  7. 7.
    Singh, R.K., Nakra, V., Pandey, H.N. and Arora, S.R. (1984) Studies on circadian periodicity of plasma, breast milk and urinary calcium in lactating Indian women. Trop. Geogr. Med. 36: 345–349.PubMedGoogle Scholar
  8. 8.
    Singh, R.K. and Bansal, A. (1987) Studies on circadian periodicity of serum and urinary urate in healthy Indians and renal stone formers. Prog. Clin. Biol. Res. 227B: 305–313.PubMedGoogle Scholar
  9. 9.
    Singh, R.K., Wu, J., Zhou, S. and Halberg, F. (1989) Circadian rhythmic human circulating cholesterol in health during fasting and on vegetarian vs. omnivorous diets. Chronobiologia 16: 183–184.Google Scholar
  10. 10.
    Singh, R.K., Bansal, A., Bansal, S.K. and Rai, S.P. (1990) Circadian rhythms of common laboratory profiles in serum and urine of healthy Indians. Progress in Clinical and Biological Research. 341B: 559–566.PubMedGoogle Scholar
  11. 11.
    Singh, R.K., Bansal, S.K., Bansal, A., Singh, A.K. and Mahdi, A.A. (1993) Circadian periodicity of urinary inhibitors of calcium oxalate crystallization in healthy Indians and renal stone formers. Eur. Urology 24: 387–392.Google Scholar
  12. 12.
    Singh, R., Singh, R.K., Mahdi, A.A., Saxena, S.P., Cornelissen, G. and Halberg, F. (2000) Circadian periodicity of urinary volume, creatinine and 5-hydroxyindoleacetic acid in healthy Indians. Life Sciences (USA) 66: 209–214.CrossRefGoogle Scholar
  13. 13.
    National Tuberculosis Association of the USA. Diagnostic standards and classification of tuberculosis. New York, 1961.Google Scholar
  14. 14.
    Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for lipid peroxides in animal tissue by thio-sarbituric acid reaction. Anal. Biochem. 95:351–358.PubMedCrossRefGoogle Scholar
  15. 15.
    McCord, J.M. and Fridovich, I. (1969) Superoxide dismutase. J. Biol. Chem. 244:6049–6055.PubMedGoogle Scholar
  16. 16.
    Aebi, H. and Suter, H. (1974) In glutathione, eds. Flohe, L. and Benhar, H.C.; Stuttgar Georg Theime, pp. 192–199.Google Scholar
  17. 17.
    Pagila, D.E. and Valentine, W.N. (1986) Studies on the qualitative and quantitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 20:150–168.Google Scholar
  18. 18.
    Halberg, F., Johnson, E.A., Nelkson, W., Runge, W. and Sothern, R. (1972) Auto-rhythmometry procedures for physiologic self measurements and their analysis. Physiology Teacher 1: 1–11.Google Scholar
  19. 19.
    Halberg, F. and Bingham, C. (1987). The scope and promise of chronobiology and biostatistics: interpenetrating, inseparable disciplines. Proc. Biopharmaceutical Section. Am. Statistical Asso., Chicago, Illinois, Au. 15–18, 11–32.Google Scholar
  20. 20.
    Bridges, A.B., Scott, N.A., McNeill, G.P., Pringle, T.H. and Belch, J.J. (1992) Circardian variation of white blood cell aggregation and free radical indices in men with ischaemic heart disease. Eur. Heart J. 13: 1632–1636.PubMedGoogle Scholar
  21. 21.
    Luo, H., Guo, H., Xiao, J. and Xue, Z. (1997) Circadian variations of plasma SOD and MDA in healthy subjects (article in Chinese). Hua, Hsiko Ta Hsuch Pao; 28: 401–403.Google Scholar
  22. 22.
    Singh, R. (2001) Biochemical studies with chronobiological approach on the role of free radical oxidants and antioxidants in the etiopathogenesis of pathological disorders. Ph.D. Thesis, Chhatrapati Shahuji Maharaj Kanpur University, Kanpur.Google Scholar
  23. 23.
    Marklund, S.L., Westman, N.G., Lundgren, E. and Ross, G. (1982) Copper and zinc containing superoxide dismutase, manganese containing superoxide dismutase, catalase and glutathione peroxidase in normal and neoplastic human cell lines and normal human tissues. Cancer Res. 42; 1955–1961.PubMedGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2004

Authors and Affiliations

  • Ranjana Singh
    • 4
  • Rajesh K. Singh
    • 1
  • Anil K. Tripathi
    • 1
  • Nikhil Gupta
    • 1
  • Ajai Kumar
    • 2
  • Anil K. Singh
    • 3
  • Abbas A. Mahdi
    • 1
  • Rajendra Prasad
    • 1
  • Raj K. Singh
    • 1
  1. 1.Departments of Biochemistry, Medicine and TB & Chest DiseasesKing George’s Medical UniversityLucknow
  2. 2.Department of BiochemistryG.S.V.M. Medical CollegeKanpur
  3. 3.Department of Community MedicineEra’s Lucknow Medical CollegeLucknow
  4. 4.Biochemistry Department, K.G.’s Medical CollegeChhatrapati Sahuji Maharaj Medical UniversityLucknow

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