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American Journal of Potato Research

, Volume 82, Issue 6, pp 433–440 | Cite as

Non-wound-induced suberization of tuber parenchyma cells: A physiological response to the wilt disease pathogenVerticillium dahliae

  • Edward C. Lulai
Article

Abstract

Verticillium spp. wilt pathogens enter the root and eventually penetrate xylem vessels of the plant where they can spread into the vascular tissue of the potato tuber. Infected tuber vessel elements often become discolored creating a serious internal tuber quality defect that prevents sale of raw product to its primary market. Despite the costly losses and disease issues created by these infections, the physiological responses to colonization of tuber vessel elements are poorly described, and a model system to study these responses in the laboratory has not been developed. The objectives of this research were to develop such a model system by determining if tuber vessel elements could be infiltrated withVerticillium spp. in a laboratory setting and if a detectable physiological response could be elicited and identified. Results demonstrated that tuber vessel elements in the model system could be infiltrated and that infiltration ofVerticillium dahliae Kleb. conidia into these vessel elements induced a suberization response on the walls of neighboring parenchyma cells. However, the walls of the infiltrated tuber vessel elements did not suberize. A similar suberization response was found in tubers that had been naturally infected byVerticillium dahliae in the field. The suberization response was histochemically determined by assessing the accumulation of suberin poly(aliphatics) and poly(phenolics). This process of internal suberization of tuber parenchyma cells occurred without induction by a wound signal. Consequently, the suberization signal was derived by introduction of the plant-pathogen into the tuber vessel elements. This simple model system provides a versatile tool to investigate the physiological responses of potato tuber to colonization of vessel elements. This is believed to be the first report for such a physiological response toVerticillium spp. in potato tuber.

Additional key words

poly(phenolic) poly(aliphatic) potato suberin Solanum tuberosum

Resumen

Los patógenos que causan marchitez (Verticillium spp.) ingresan a la raíz y eventualmente penetran el xilema, desde donde pueden diseminarse al tejido vascular del tubérculo de papa. Los elementos de los vasos infectados a menudo se decoloran, produciéndose un daño interno que atenta contra la calidad del tubérculo y que impide su comercialización al estado natural en el mercado primario. A pesar de las pérdidas cuantiosas a causa de estas infecciones, las respuestas fisiológicas a la colonización de los elementos vasculares del tubérculo han sido deficientemente descritas y no se ha desarrollado un sistema modelo para estudiarlas. Los objetivos de esta investigación fueron desarrollar un sistema modelo para determinar si los elementos vasculares del tubérculo pueden ser infiltrados conVerticillium spp. en un ambiente de laboratorio y si sería posible producir e identificar la respuesta fisiológica. Los resultados demostraron que los elementos de los vasos en el sistema modelo pueden ser infiltrados y que la infiltración de conidias deVerticillium dahliae Kleb indujeron una respuesta de suberización sobre las paredes de las células del parénquima vecino. Sin embargo, las paredes del sistema vascular de las células infiltradas no suberizaron. Una respuesta similar de suberización se encontró en tubérculos naturalmente infectados porV. dahliae en el campo. La respuesta de suberización ha sido histoquímicamente determinada, evaluando la acumulación de suberina polialifática y polifenólica. Este proceso de suberización interna de las células del tubérculo se produjo sin la inducción de una señal de herida. Consecuentemente la señal de suberización se derivó de la introducción del patógeno en los elementos del sistema vascular. Este simple sistema modelo proporciona una herramienta versátil para investigar las respuestas fisiológicas del tubérculo de papa a la colonización de los elementos del sistema vascular. Se cree que este es el primer reporte sobre la respuesta fisiológica de los tubérculos de papa aVerticillium spp.

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.USDA-ARS, Northern Crop Science LaboratoryFargoUSA

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