Indian Journal of Clinical Biochemistry

, Volume 17, Issue 2, pp 33–43 | Cite as

Hypoglycemic, hypolipidemic and antioxidant properties of combination ofCurcumin fromCurcuma longa, Linn, and partially purified product fromAbroma augusta, Linn. in streptozotocin induced diabetes

  • Halim Eshrat M. Ali Hussain


Dietary spice components ofCurcuma longa andAbroma augusta have been screened for their protective effect against reactive oxygen species induced lipid peroxidation. They have been found to be efficient antioxidant when administered in combination. The purpose of the study was to investigate the effect of oral administration (300mg/Kg) of the aqueous extract of turmeric whose active ingredient isCurcumin andAbromine powder as a hypoglycemic agent mixed with diet. The effect of this aqueous extract on blood glucose, lipid peroxidation (LPO) and the antioxidant defense system in rat tissues like liver, lung, kidney and brain was studied for 8 weeks in streptozotocin induced diabetic rats. The administration of an aqueous extract of turmeric and abromine powder resulted in a significant reduction in blood glucose and an increase in total haemoglobin. The aqueous extract also resulted in decreased free radical formation in the tissues studied.

The decrease in thiobarbituric acid reactive substances (TBARS) and increase in reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) clearly showed the antioxidant property of the mixture. It is suggested that these changes initially counteract the oxidative stress in diabetes however, a gradual decrease in the antioxidative process may be one of the factors which results in chronic diabetes. These results indicate that the mixture of the two plants have shown antidiabetic activity and also reduced oxidative stress in diabetes. A combination ofAbroma augusta and Curcuma longa also restored the other general parameters in diabetic animals. The results were statistically analyzed and indicated that combination of herbal extracts showed better efficacy as compared to individual herbal plant extracts used.


Abroma augusta Curcumin diabetes mellitus hypoglycemia hypolipidemic effect lipid peroxidation antioxidants 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Melinda A., (1988) Non insulin dependent diabetes mellitus treatment with sulphonylureas in Clinical Endocrinology and metabolism. Des natures, M. and Hale, P. Balliere- Tindall, London.; P, 443–453.Google Scholar
  2. 2.
    World Health Organization. (1980) Expert committee diabetes mellitus. Technical Report Second Series. No. 646, WHO, Geneva, p. 61.Google Scholar
  3. 3.
    Satyavati, G. V. and Gupta, A. K. (1987) Medicinal plants of India eds Indian Council, New Delhi: Vol. II. Shankar, TNB; Shanta N. V., Ramesh. H.P.; Murthy I.A.S., Murthy V.S., 1987 Ind. J. Exp. Biol. 1980; 18, 73.Google Scholar
  4. 4.
    Oubre, A.Y., Carlson, T.J., King, S.R. and Reaven, G.M. (1970) from plant to patient, an Ethanomedical approach to the identification of new drugs for the treatment of NIDDM. Diabetologia; 40 (5): 614–617.Google Scholar
  5. 5.
    Shnkar, TNB; Shanta N.V., Ramesh, H.P.; Murthy I.A.S. Murthy V.S. (1980). Toxicity Studies on Turmeric (Cuecuma longa): Acute Toxicity studies in rats, Guineapigs&Monkeys. Ind. J. Exp. Bio. 1980; 18 (1): 73–75.Google Scholar
  6. 6.
    Shih, C.A., Lin J.K. (1980) Inhibition of 8-Hydroxydeoxyguanosive formation by curcumin in mouse fibroblast cells. carcinogenesis; 14 (4) 709–712CrossRefGoogle Scholar
  7. 7.
    Tank, R., Sharma, N., Sharma, I., Dixit, VP (1989) Anti-diabetic activity ifC.longa in alloxan Induced diabetic rats—Indian drugs: 27 (11) 587–589.Google Scholar
  8. 8.
    Hakino, H. (1985) Anti hepatotoxic activity of crude drugs. Yakugaku Zasshi. 105 (2) 109–118.Google Scholar
  9. 9.
    Soudamini, K. K., Kuttan, R. (1989) Inhibition of chemical carcinogenesis by Curcumins. J. Ethnopharmacology; (27): 227–233.CrossRefGoogle Scholar
  10. 10.
    Hussein, H.E.M.A., Jamil, K. and Rao, M. (2001) Preliminary studies on the Hypoglycemi effect ofAbroma augusta in alloxan diabetic rats. Ind. J. clin. Biochem. 16 (1). 77–80.Google Scholar
  11. 11.
    Poliodoro, G.D.T., Arduini, R.G.L.A. and Federici, G. (1984) Superoxide dissimulates, reduced Glutathione and TBA-reactive products in erythrocytes of patients with multiple sclerosis. Int. J. Biochem. 16 (50): 505–509.CrossRefGoogle Scholar
  12. 12.
    Hiroshi, O., Nobuko, O. and Kunio, V. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 5, 351–358.Google Scholar
  13. 13.
    Wendell, A. (1981) Glutathione peroxidase. In: Methods in Enzymology, Ed. Jakoby, W.B. Academic Press, New York, USA Vol. (77): P. 327–333.Google Scholar
  14. 14.
    Hussein, H.E.M.A., Jamil, K. and Rao, M. (2001) Hypoglycemic, hypolipidemic and antioxidant properties of tulsi (Ocimumsanctum Linn) on streptozotocin induced diabetes in rats. Ind. J. clin. Biochem. 16 (2). 190–194.CrossRefGoogle Scholar
  15. 15.
    Salimath BP, Sundaresh C.S. Srinivas L. (1985) Dietary compounds inhibit lipid peroxidation in erythrocyte membrane. Nut Res; (6): 1171–1178.CrossRefGoogle Scholar
  16. 16.
    Huang MT, Smart RC, Wong CQ, Conney AH. (1988) inhibitory effect of Curcumin, chlorogenicacide. Coffeic acid, and ferric acid on tumour promotion in mouse skin by 12-0-tertradecanoyl. phorobol.; 13(48): 5941–5946.Google Scholar
  17. 17.
    Betteridge J. (1997) Lipid disorders in diabetes mellitus, in: J.C. Pickup, G. Williams (Eds.) textBook of Diabetes, sec. Ond ed., Blackwell Science. London; PP, (31). 1–55.Google Scholar
  18. 18.
    Brown G.B., Xue-Qiao, Sacco, D.E. and Alberts, J.J. (1993) Lipid lowering and plaque regression. New insights into prevention of plaque disruption and clinical events in coronary disease, Circulation; (87): 1781–1791.PubMedGoogle Scholar
  19. 19.
    Karunanayake, E. H., Tendon, K. H., (1993) Search of novel hypoglycemic agents from medicinal plants, in: AK Sharma (Ed.) Diabetes Mellitus and Its Complications, An update, macmillan India LTD, New Delhi, India, pp. 192–205.Google Scholar
  20. 20.
    Mata P, Alonso R, Lopez-Fare A, Orders JM, Lahore C, Caramel C, Codices R, Blanquez E, De, Ova M (1996). Effect of dietary fat saturation on LDL oxidation and monocyte adhesion too human Endothelial cells in vitro. Arterioscler Thromb Vasc Biol; 16: 1347–1355.PubMedGoogle Scholar

Copyright information

© Association of Clinical BIochemists of India 2002

Authors and Affiliations

  • Halim Eshrat M. Ali Hussain
    • 1
  1. 1.National Chemical LaboratoryPuneIndia

Personalised recommendations