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Indian Journal of Clinical Biochemistry

, Volume 14, Issue 2, pp 220–228 | Cite as

Comparative evaluation of ultramicro—and macro-chemo enzyme based assays of glucose, cholesterol and triglycerides

  • Ganesa YogeeswaranEmail author
  • Charles E. Jebaraj
  • Venkataraman Sriram
Methods

Abstract

A comparison of the absorbance, enzyme/substrate concentration, reaction efficiency and sensitivity has been made for enzyme-based clinical chemistry assays, using a conventional colorimeter versus a strip-microwell reader, in order to establish the value of ultra-microchemical procedure, with reaction volume 87 μl (light path length=0.25 cm). By utilizing commercial kits available for the quantitation of serum glucose, cholesterol and triglycerides, it has been established that the micro method is highly cost effective (9–30 fold), reproducible and sensitive. Comparison of blood drawn by a finger prick (capillary) and venipuncture for normal and pathological specimens show reproducibility between different laboratory technologists and in reference with the values reported by an accredited reference laboratory. Since the micro method uses very little serum, it is most suitable for analyses of small smaples, from large population-based field trials. However, the assay range has to be titrated for each commercial kit to establish the enzyme/substrate equivalence.

Key words

Micro-method Blood Chemistry Sensitive Assay Capillary-venous Blood Colorimetry 

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Copyright information

© Association of Clinical Biochemists of India 1999

Authors and Affiliations

  • Ganesa Yogeeswaran
    • 1
    Email author
  • Charles E. Jebaraj
    • 1
  • Venkataraman Sriram
    • 1
  1. 1.Department of Medical BiotechnologyAffiliated to University of Madras, Tamilnad Hospital Academic Trust-Research CouncilChennaiIndia

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