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Indian Journal of Clinical Biochemistry

, Volume 13, Issue 2, pp 55–62 | Cite as

Interferences in clinical chemistry analysis

  • K. S. S. Saibaba
  • M. Vijaya Bhaskar
  • P. V. L. N. Srinivasa Rao
  • G. V. Ramana
  • K. V. Dakshinamurty
Review

Abstract

Analyses of body fluids in clinical chemistry laboratory are subject to a number of interferences that affect the analytical accuracy. The interferents arise from exogenous sources like drugs and additives as well as such endogenous sources like lipemia, hemolysis and icteria. Our studies demonstrate matrix interference in the form of analytical blas between serum and aqueous matrix calibrators. The clinical chemist should constantly be aware of this factor. Correction of interferences is recommended as an integral part of the quality assurance program.

Key words

Interference Bichromatic analysis Matrix effects Hemolysis Quality assurance 

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References

  1. 1.
    Narayanan, S. (1996) Pre and post analytical errors. Ind. J. Clin. Biochem. 11 (1), 7–11Google Scholar
  2. 2.
    Narayanan, S. (1996) Pre and post analytical errors in lipid determination. Ind. J. Clin. Biochem. 11 (1), 12–16.Google Scholar
  3. 3.
    Kanagasabapathy, A.S., Swaminathan, S. and Selvakumar, R. (1996). Quality control in clinical biochemistry. Ind. J. Clin. Biochem. 11 (1) 17–25.Google Scholar
  4. 4.
    Kroll, M.H. and Elin, R.J. (1994) Interference with Clinical Laboratory Analyses. Clin. Chem. 40 (11), 1996–2005.PubMedGoogle Scholar
  5. 5.
    Munzenberger, P. and Emmanuel, S. (1971). The incidence of drug diagnostic test interferences in outpatients Am. J. Hosp. Pharm. 28 786–91.PubMedGoogle Scholar
  6. 6.
    Murphy, J.L., Hurt, T.L., Griswold, W.R., Peterson, B.M., Rodrate, A. and Krouse, H.F. (1989) Interference with creatinine concentration measurement by high dose furosemide infusion. Crit. Care Med. 17, 889–890.PubMedCrossRefGoogle Scholar
  7. 7.
    Saah, A.J., Koch T.R. and Drusano, G.L. (1982) Cefoxitin falsely elevates creatinine levels. J. Am. Med. Assoc. 247, 205–206.CrossRefGoogle Scholar
  8. 8.
    Siff K.S. and Finkler, A.E. (1988) False positive barbiturate test in urine owing to phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin (Tech Brief) Clin. Chem. 34, 1359–1360.PubMedGoogle Scholar
  9. 9.
    Siest, G., Dawkins, S.J. and Galteau, M.M. (1983) Drug effects on laboratory tests. J. Pharm. Biomed. Anal. 1, 247–257.PubMedCrossRefGoogle Scholar
  10. 10.
    Young, D.S., Pestaner, L.C. and Gibberman, V. (1975) Effect of drugs on clinical laboratory tests. Clin. Chem. 21, 1D-432D.PubMedGoogle Scholar
  11. 11.
    Lasky, F.D. (1993) Achieving accuracy for routine clinical chemistry methods. Arch. Pathol. Lab. Med. 117, 412–419.PubMedGoogle Scholar
  12. 12.
    Ross, J.W., Myers, G.L., Gilmore, B.F., Cooper, G.R., Naito, H.R. and Eckfeldt, J. (1993) Matrix effects and the accuracy of cholesterol analysis. Arch. Pathol. Lab. Med. 117, 393–400.PubMedGoogle Scholar
  13. 13.
    Saibaba, K.S.S., Srinivasa Rao, P.V.L.N., Ramana, G.V., Kumar, E.G.T.V., and Tripathi, R.L. (1995) Analytical bias due to calibrator matrix effects. Ind. J. Clin. Biochem. 10 (2), 112–115.CrossRefGoogle Scholar
  14. 14.
    Henry R.J., Sobel, C. and Berkman, S. (1957) On the determination of “pancreatic lipase” in serum. Clin. Chem. 3, 77–89.PubMedGoogle Scholar
  15. 15.
    Pudek, M.R. and Nanji, A.A. (1983) Antibody interference with biochemical tests and its clinical significance. Clin. Biochem. 16, 275–80PubMedCrossRefGoogle Scholar
  16. 16.
    Kaplan, L.A. and Pesce, A.J. (1989) Interferences in chemical analysis In: Clinical Chemistry-Theory, analysis and correlation. Eds. Kaplan L.A. and Pesce, A.J. The C.V. Mosby Company. St. Louis, USA, 2nd edn. p.808–819.Google Scholar
  17. 17.
    Fischbach, F.T. (1984) A manual of laboratory diagnostic tests. J.B. Lippincot Company, Philadelphia, 2nd edn. p. 823–847.Google Scholar

Copyright information

© Association of Clinical Biochemists of India 1998

Authors and Affiliations

  • K. S. S. Saibaba
    • 1
  • M. Vijaya Bhaskar
    • 2
  • P. V. L. N. Srinivasa Rao
    • 1
  • G. V. Ramana
    • 1
  • K. V. Dakshinamurty
    • 3
  1. 1.Department of BiochemistrySri Venkateswara Institute of Medical Sciences (SVIMS)Tirupati
  2. 2.S.V. Medical CollegeTirupati
  3. 3.Department of NephrologyNizam’s Institute of Medical SciencesHyderabad

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