Indian Journal of Clinical Biochemistry

, Volume 18, Issue 2, pp 48–53

Serodiagnosis of tuberculosis using two ELISA systems

  • Swati Banerjee
  • Sonika Gupta
  • Niraj Shende
  • Satish Kumar
  • Bhaskar C. Harinath
Article

Abstract

Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H37Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.

Key Words

ELISA ES-31 serodiagnosis tuberculosis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bothamely, G. H. (1995) Serological diagnosis of tuberculosis. Eur. Respir. J. 8 (suppl 20), 6768–6885.Google Scholar
  2. 2.
    Charpin, D., Herbault, H., Gevaudan, M. J., Saadjian, M., De Micco, P. (1990) Value of ELISA using A60 antigen In the diagnosis of Active Pulmonary tuberculosis. Am. Rev. Respir. Dis. 42, 380–384.Google Scholar
  3. 3.
    Al-Hajjaj, M. S., Gad-El-Rab, M. O., Al-orainey, I. O., Al-Kassimi, F. A. (1999) Improved sensitivity for detection of tuberculosis cases by a modified Anda-TB ELISA test. Tub. Lung Dis. 79 (3), 181–185.CrossRefGoogle Scholar
  4. 4.
    Sachan, A. S., Gupta, R. K., Gupta, D., Gautam, K. D. (1994) Diagnostic significance of antigen A-60 by ELISA test in pulmonary tuberculosis. Indian J. Tub. 41, 239.Google Scholar
  5. 5.
    Gupta, S., Kumari, S., Banwalikar, J. N., Gupta, S. K. (1995). Diagnostic utility of the estimation of mycobacterial antigen A60 specific immunoglobulins IgM, IgA and IgG in the sera of cases of adult human tuberculosis. Tub. Lung Dis. 76, 418–424.CrossRefGoogle Scholar
  6. 6.
    Verbon, A. (1994) Development of a serological test for TB. Problems and potential. Trop. Geogr. Med. 46 (5), 275–279.PubMedGoogle Scholar
  7. 7.
    Luh, K. T., Yu, C. J., Yang, P. C., Lee, L. N. (1996) Tuberculosis antigen A60 serodiagnosis in tuberculosis infection: application in extra pulmonary and smear negative pulmonary tuberculosis. Respirology 1 (2), 145–51.PubMedCrossRefGoogle Scholar
  8. 8.
    Alifano, M., De Pascalis, R., Sofia, M., Faraone, S., Del Pezzo, M., Covelli, I. (1998) Detection of IgG and IgA against the mycobacterial antigen A60 in patients with extra pulmonary tuberculosis. Thorax 53 (5), 377–380.PubMedCrossRefGoogle Scholar
  9. 9.
    Yu, C. J.; Yang, P. C., Hsieb, R. P., Lun, K. T. (1992) Evaluation of the A60 IgG ELISA serodiagnostic test for tuberculosis in Taiwan. J. Formos. Med. Assoc. 91 (6), 614–619.PubMedGoogle Scholar
  10. 10.
    Gevaudan, M. J., Bollet, C., Charpin, D., Mallet, M. W. (1992). Serological response of tuberculosis patients to antigen A60 of BCG. Eur. J. Epidemiol. 8, 666–676.PubMedCrossRefGoogle Scholar
  11. 11.
    Nair, E. R., Banerjee, S., Kumar, S., Reddy, M. V. R., Harinath, B. C. (2001). Purification and characterization of a 31kDa mycobacterial excretory-secretory antigenic protein with a diagnostic potential in pulmonary tuberculosis. Indian J. Chest Dis. Allied Sci. 43, 81–90.PubMedGoogle Scholar
  12. 12.
    Daniel, T. M., Debanne, S. M. (1987). The Serodiagnosis of tuberculosis and other mycobacterial disease by enzyme linked immunosorbent assay. Am. Rev. Respir. Dis. 135, 1137–1157.PubMedGoogle Scholar
  13. 13.
    Mathai, A., Radhakrishnan, V. V., Sehgal, S. (1990) IgG antibody to M. Tuberculosis antigen 5 in cerebrospinal fluid and its diagnostic application in tuberculous meningitis. Indian J. Exp. Biol. 28: 816–820.PubMedGoogle Scholar
  14. 14.
    Reggiardo, A., Vazques, E., Schnaper, L. (1980) ELISA test for antibodies against mycobacterial glycolipids. J. Immunol. Meth. 34, 55–60.CrossRefGoogle Scholar
  15. 15.
    Sada, E., Ferguson, L. E., Daniel, T. M. (1990) An ELISA for the serodiagnosis of tuberculosis using a 30,000-Da native antigen of Mycobacterium tuberculosis. J. Infect. Dis. 162, 928–931.PubMedGoogle Scholar
  16. 16.
    Thongkrajai, P., Lulitanon, V., Chamnavanki, C. (1989). Improved ELISA with immunosorbent purified mycobacterial antigens for serodiagnosis of tuberculosis. J. Med. Microbiol. 30, 101–104.PubMedCrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2003

Authors and Affiliations

  • Swati Banerjee
    • 1
    • 2
  • Sonika Gupta
    • 1
    • 2
  • Niraj Shende
    • 1
    • 2
  • Satish Kumar
    • 1
    • 2
  • Bhaskar C. Harinath
    • 1
    • 2
  1. 1.Jamnalal Bajaj Tropical Disease Research CentreMahatma Gandhi Institute of Medical SciencesWardhaIndia
  2. 2.Department of BiochemistryMahatma Gandhi Institute of Medical SciencesWardhaIndia

Personalised recommendations