Indian Journal of Clinical Biochemistry

, Volume 16, Issue 2, pp 203–206 | Cite as

Assay of tubercular antibody, circulating free and immune complexed antigen in the diagnosis of pulmonary tuberculosis

  • Swati Banerjee
  • E Raji Nair
  • Satish Kumar
  • M. V. R. Reddy
  • Bhaskar C. Harinath
Brief Communications

Abstract

Analysis of tubercular antibody, circulating free and immune complexed antigen (CIC-Ag) was done in confimed pulmonary tuberculosis sera by ELISA, using ES-31 antigen and affinity purified anti ES-31 antibody. Twenty three of 25 (92%) tuberculosis sera were positive for IgG antibody to ES-31 antigen. Using anti ES-31 antibody, free tubercular antigen could be detected in 20 of 25 (80%) cases whereas circulating immune complexed antigen (CIC-Ag) in 18 of 25 (72%) cases by sandwich ELISA. Of the two sera showing absence of antibody, one showed presence of free and CIC-Ag whereas the other showed the presence of free antigen. Thus antigen assay may be used as an adjunct tool for confirmation of pulmonary tuberculosis.

Key words

Antibody Antigen ELISA ES-31 Antigen Immunodiagnosis Pulmonary tuberculosis 
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Copyright information

© Association of Clinical Biochemists of India 2001

Authors and Affiliations

  • Swati Banerjee
    • 1
  • E Raji Nair
    • 1
  • Satish Kumar
    • 1
  • M. V. R. Reddy
    • 1
  • Bhaskar C. Harinath
    • 1
  1. 1.Jamnalal Bajaj Tropical Disease Research Centre and Department of BiochemistryMahatma Gandhi Institute of Medical SciencesSevagram, WardhaIndia

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