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American Potato Journal

, Volume 44, Issue 7, pp 249–254 | Cite as

Use of molecular gel column to prepare potato virus X inocula for antisera

  • James Munro
Article

Summary

A simple technique has been developed to obtain purified potato virus X in physiological saline solution by passage through a gel column. The virus solution is the concentrated by dialysis with polyethylene glycol for use in intravenous injections for the production of potato virus X-immune rabbit antiserum.

Keywords

AMERICAN Potato Journal Potato Virus Dialyse Tubing Blue Dextran Virus Inoculum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Resumen

Se ha desarrollado una técnica sencilla para obtener el virus X de patata purificado en una solución salina fisiólógica por pasarse por una columna de gelatina. Luego, se concentra la solución del virus por diálisis con el glicol polietileno para usarse en intervenciones in travenosas para la producción del antisuero de conejo inmune del virus X de patata.

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Literature Cited

  1. 1.
    Bawden, F. C. and A. Kleczkowski. 1948. Variations in the properties of potato virus X and their effects on its interaction with ribo-nuclease and proteolytic enzymes. J. Gen. Microbiol. 2: 173–185.Google Scholar
  2. 2.
    Kohn, J. 1959. A simple method for the concentration of fluids containing proteins. Nature 183: 1055.PubMedCrossRefGoogle Scholar
  3. 3.
    Loring, Hubert S. and W. M. Stanley. 1937. Isolation of crystalline tobacco mosaic virus protein from tomato plants. J. Biol. Chem. 117: 733.Google Scholar
  4. 4.
    Munro, James. 1954. Maintenance of virus X-free potatoes. Amer. Potato J. 31: 73–82.CrossRefGoogle Scholar

Copyright information

© Springer 1967

Authors and Affiliations

  • James Munro

There are no affiliations available

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