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American Potato Journal

, Volume 66, Issue 10, pp 669–684 | Cite as

Variability in potato tissue culture

  • A. Karp
  • M. G. K. Jones
  • D. Foulger
  • N. Fish
  • S. W. J. Bright
Article

Abstract

Tissue culture systems in potato (Solanum tuberosum L.) that involve a callus phase, such as regeneration of plants from protoplasts or expiants, can be accompanied by phenotypic variability, or somaclonal variation. The extent of this variability appears to be greatest in protoplast-derived potato plants. Cytological studies have shown that some of the variation observed in regenerated potato plants arises from numerical and structural chromosome variation. Aneuploidy is particularly a problem in regeneration from protoplasts. Factors affecting the degree of chromosome instability include the genotype and ploidy of the starting material, the regeneration system adopted, the source of the plant cells and the composition of the tissue culture media. Studies at the molecular level have indicated that changes in the potato genome, other than those associated with chromosomes, also occur. The implications of somaclonal variation in potato, particularly with regard to its potential usefulness for plant breeding are discussed.

Keywords

Potato Plant AMERICAN Potato Journal Solanum Tuberosum Potato Cultivar Somaclonal Variation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Compendio

Los sistemas de cultivo de tejidos de papa (Solanum tuberosum L.) que incluyen una fase de callo, tal como la regeneración de plantas a partir de protoplastos o tejidos vivos de plantas, pueden ser acompañados por una variación del fenotipo, o variación somaclonal. El grado de esta variación parece ser máximo en plantas de papa obtenidas de protoplastos. Los estudios citológicos han demostrado que algunas de las variaciones observadas en las plantas regeneradas surgen de una variación en el número y estructura de los cromosomas. La aneuploidia es particularmente un problema en la regeneración a partir de protoplastos. Los factores que afectan el grado de inestablilidad de los cromosomas incluyen el genotipo y la ploidia del material inicial, el sistema de regeneracion adoptado, la fuente de células vegetales y la composición del medio de cultivo para los tejidos. Estudios al nivel molecular han indicado que también ocurren cambios en el genoma de papa, diferentes a los asociados con los cromosomas. Se discuten las implicancias de la variación somaclonal en papa, principalmente en lo referente a su uso potencial en el mejoramiento de plantas.

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Copyright information

© Springer 1989

Authors and Affiliations

  • A. Karp
    • 1
  • M. G. K. Jones
    • 1
  • D. Foulger
    • 1
  • N. Fish
    • 1
  • S. W. J. Bright
    • 1
  1. 1.Biochemistry DepartmentRothamsted Experimental StationHarpendenEngland

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