American Potato Journal

, Volume 60, Issue 2, pp 99–108 | Cite as

Stage of development of leaf and tuber tissue of the potato plant influences the titer of potato virus M

  • S. M. Tavantzis
Article
  • 22 Downloads

Abstract

The enzyme-linked immunosorbent assay (ELISA) was adapted for the detection of potato virus M (PVM). Detectability of PVM was influenced by the stage of plant growth and that of the plant part assayed. In young potato plants, one week prior to flowering, PVM titer was relatively high (0.77 A405nm units) in the basal leaves and barely detectable (0.09 A405 nm units) in the top leaves. In mature plants, two weeks prior to harvesting, top leaves contained more virus (0.34 A405 nm units) than middle (0.24 A405nm units) or basal leaves (0.15 A405 nm units). In rapidly growing tubers PVM content was higher (1.57 A405 nm units) than that in fully grown tubers (0.71 A405nm units) whereas the virus was not detectable in the mother tuber four weeks following planting. PVM was reliably detected when disks from intact leaves were substituted for leaf extracts as the test sample

Key Words

Potato virus M detection ELISA plant growth stage plant organ leaf discs 

Resumen

La técnica serológica de inmunoabsorción con conjugados enzimáticos (ELISA) fue adaptada para la detección del virus M de la papa (PVM). La detectabilidad de PVM estuvo influenciada por el estado de crecimiento de la planta y de la parte de la planta examinada. En plantas jovenes de papa, una semana antes de la floración, el contenido de PVM fue relativamente alto (A405nm = 0.77) en las hojas basales y escasamente detectables (A405nm = 0.09) en las hojas del brote apical. En plantas maduras, dos semanas antes de la cosecha, las hojas del brote apical contenían más virus (A405nm = 0.34) que las hojas medias (A405nm = 0.24) o basales de la planta (A405nm = 0.15). En tubérculos en formación el contenido de PVM fue más alto (A405nm = 1.57) que en tubérculos completamente maduros (A405nm = 0.71) mientras que el virus no fue detectable en los tubérculos madres cuatro semanas después de plantados. PVM fue detectado con seguridad en discos de hojas intactas en vez de extractos de hojas

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Copyright information

© Springer 1983

Authors and Affiliations

  • S. M. Tavantzis
    • 1
  1. 1.Department of Botany and Plant PathologyUniversity of MaineOrono

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