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American Journal of Potato Research

, Volume 82, Issue 3, pp 231–239 | Cite as

Investigations on the role of acid invertase and UDP-glucose pyrophosphorylase in potato clones with varying resistance to cold-induced sweetening

  • Marian J. McKenzieEmail author
  • Joseph R. Sowokinos
  • Irene M. Shea
  • Sanjay K. Gupta
  • Rebecca R. Lindlauf
  • John A. D. Anderson
Article

Abstract

Fifteen potato (Solanum tuberosum L.) clones with varying resistance to cold-induced sweetening were analysed for vacuolar acid invertase (AcInv, EC 3.2.1.26) and UDP-Glc pyrophosphorylase (UGPase; EC 2.7.7.9) activities related to their ability to accumulate sugars following cold storage (4 C). The UGPase isozyme profiles for each clone were also determined. Immunoblot analysis demonstrated a 55 kD protein, in seven of the 15 clones, that reacted with UGPase antisera in addition to the 53 kD UGPase subunit previously reported. The UGPase activity of these clones was significantly lower than that of the “single subunit” clones. Basal AcInv activity showed a positive correlation to the Glc:Suc ratio across the clones that was moderately significant before and after cold temperature storage. The activity of UGPase, which limits the rate of Suc formation, was of secondary importance in limiting the rate of hexogenesis when vacuolar AcInv activity was excessive. It is suggested that with the potato clones from this breeding program that AcInv (and its inhibitor) plays a dominant role in the hexogenic pathway by regulating the hexose:Suc ratio. The finding of a new protein that is reactive with UGPase antisera may prove to play an important function in the regulation of Suc formation in potatoes.

Additional Key Words

cold storage invertase inhibitor processing sugars 

Abbreviations

CIS

cold-induced sweetening

Fru

fructose

FW

fresh weight

Glc

glucose

Inv

invertase

AcInv

acid invertase

SPS

sucrose phosphate synthase

Suc

sucrose

TBS

Tris-buffered saline

UGPase

uridine-5’-diphosphoglucose pyrophosphorylase

UDP-Glc

uridine-5’-diphosphoglucose

Resumen

Quince clones de papa (Solanum tuberosum L.) con varios grados de resistencia al endulzamiento inducida por el frío, fueron analizados para invertasa ácida (AcInv; EC 3.2.1.26) vacuolar y actividades de UDP-Glc fosforilasa (UGPasa; EC 2.7.7.9), relacionadas con su habilidad para acumular azucares después de su almacenamiento en frío (4 C). También se determinaron para cada clon los perfiles de la isoenzima UGPasa. El análisis inmunoblot demostró en siete de los 15 clones una proteína kD que reaccionó con el antisuero UGPasa cuando se agregó a la subunidad 53 kD UGPasa previamente reportadas. La actividad de UGPasa en estos clones fue significativamente mas baja que la de los clones de la “subunidad simple.” La actividad basal del AcInv demostró una correlación positiva con la proporción Glc: Sac en los clones que fueron moderadamente significativos antes y después del almacenamiento a baja temperatura. La actividad de la UGPasa la cual imita la tasa de formación de sacarosa, fue de importancia secundaria en limitar la tasa de hexogénesis cuando la actividad vacuolar del AcInv fue excesiva. Se sugiere que con los clones de papa de este programa, el AcInv (y su inhibidor) juega un rol dominante en la vía hexogénica regulando la proorción hexosa: sacarosa. El hallazgo de una nueva proteína que reacciona con el antisuero UGPasa, puede evidenciar que esta juega una función importante en regular la formación de sacarosa en papa.

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Copyright information

© Springer 2005

Authors and Affiliations

  • Marian J. McKenzie
    • 1
    Email author
  • Joseph R. Sowokinos
    • 2
  • Irene M. Shea
    • 2
  • Sanjay K. Gupta
    • 2
  • Rebecca R. Lindlauf
    • 3
  • John A. D. Anderson
    • 4
  1. 1.Food Industry Science CentreThe New Zealand Institute for Crop & Food Research LimitedPalmerston NorthNew Zealand
  2. 2.Department of Horticultural ScienceUniversity of MinnesotaSt. PaulUSA
  3. 3.USDA-ARS Potato Research WorksiteEast Grand ForksUSA
  4. 4.Pukekohe Research CentreThe New Zealand Institute for Crop & Food Research LimitedPukekoheNew Zealand

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