American Potato Journal

, Volume 69, Issue 7, pp 423–435 | Cite as

Differences in free and protein-bound tyrosine among potato genotypes and the relationship to internal blackspot resistance

  • Dennis L. Corsini
  • Joseph J. Pavek
  • Bill Dean
Article

Abstract

Twelve potato clones were selected to represent the full range of internal blackspot response in order to determine the relationships between tuber protein, free tyrosine, and blackspot susceptibility. The blackspot reaction for each clone was consistent over five growing seasons, including tubers grown over a normal season (mature), and short season (immature) during one year. The blackspot index, determined by either an abrasive peel test or an impact bruise test, was highly correlated with the tyrosine content of the tubers (r = 0.90 p = 0.001 for the means of each clone over five location-years). Tubers with free tyrosine levels below 4 μmole/g dry weight consistently showed a resistant blackspot response. The relationship between tyrosine and blackspot susceptibility was also found in stolon and bud ends from five of the clones which represented the extremes of blackspot reaction and genetic diversity. Bud end samples of each of the clones had lower tyrosine content and a corresponding reduction in blackspot compared with stolon ends. Phenols, other than tyrosine, showed no consistent relationship to the blackspot reaction.

There was a very high negative correlation between free tyrosine and estimated protein-bound tyrosine. R values ranged from −0.85 to −0.97 (p = 0.001) for mature tubers of the 12 clones over 4 growing seasons. Total tyrosine (free, plus protein-bound) remained relatively constant. There were no significant differences in mean total tyrosine content among the 12 clones over five location-years of testing; and there were no significant differences among the five growing seasons except for the short season (immature) tubers which were 14% lower in total tyrosine content. These results indicate a remarkably constant level of total tyrosine production in the twelve clones studied, that represented diverse genetic backgrounds. Mature tubers of all genotypes contained 26 ± 1 μmole/g dry weight total tyrosine. Partitioning of tyrosine between tuber protein and the free amino acid pool varied with genotype and appeared to be a major determinate of blackspot resistance.

Additional Key Words

Potato protein potato amino acids Solanum tuberosum 

Compendio

Se seleccionaron doce clones de papa para representar el rango completo de la respuesta de la mancha negra interna para determinar la relación entre la proteína, la tiroxina libre y la susceptibilidad a la mancha negra. La reacción a la mancha negra para cada clon fue consistente durante cinco temporadas, incluyendo tubérculos que crecieron en una temporada normal (maduros) y en una temporada corta (inmaduros) durante un año. El índice de mancha negra, determinado ya sea por una prueba de peladura con abrasivo o por una prueba de daño por impacto, estuvo ampliamente correlacionado con el contenido de tiroxina de los tubérculos (r=0, 90 p = 0,001 para los promedios de cada clon en cinco anos-localidad). Los tubérculos con niveles por debajo de 4 μmole/g de peso seco mostraron consistentemente una respuesta resistente a la mancha negra. La relación entre la tiroxina y la susceptibilidad a la mancha negra también fue encontrada en los estolones y puntas de las yemas de cinco de los clones que representaron los extremos de la rección a la mancha negra y diversidad genética. Muestras de las puntas de las yemas de cada uno de los clones tuvieron contenidos más bajos de tiroxina y la reducción correspondiente en mancha negra en comparación con las puntas de los estolones. Otros fenoles diferentes a la tiroxina no mostraron una relación consistente a la reacción a la mancha negra.

Hubo una correlación negativa muy alta entre la tiroxina libre y el estimado de tiroxina ligada a proteína. Los valores de R variaron de −0,85 a −0.97 (p=0,001) para los tubérculos maduros de los 12 clones durante cuatro temporadas. La tiroxina total (libre, más ligada a proteína) permanecieron relativamente constantes. No hubieron diferencias significativas en el contenido promedio de tiroxina total entre los 12 clones durante cinco de los años-localidad y tampoco las hubo entre las cinco temporadas excepto para los tubérculos de la temporada corta (inmaduros) que tuvieron 14% menos de contenido total de tiroxina. Estos resultados indican un nivel constante notable de producción de tiroxina total en los doce clones estudiados y que representaban bases genéticas diversas. Los tubérculos maduros de todos los genotipos contuvieron 26 ± 1 μmole/g de peso seco de tiroxina total. La distribución de la tiroxina entre la proteína del tubérculo y el (pool) de amino ácidos libres varió con el genotipo y pareció ser un factor determinante de la resistencia a la mancha negra.

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Literature Cited

  1. 1.
    Bradford, N.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein dye binding. Anal Biochem 72:248–254.PubMedCrossRefGoogle Scholar
  2. 2.
    Desborough, S.L. and C.J. Weiser. 1974. Improving potato protein I. Evaluation of selection techniques. Am Potato J 51:185–196.Google Scholar
  3. 3.
    Gubb, I., J.A. Callow, R.M. Faulks and M.T. Jackson. 1989. The biochemical basis for the lack of enzymatic browning in the wild potato speciesSolanum hjertingii Hawkes. Abstract. Am Potato J 66:522.Google Scholar
  4. 4.
    Hughes, J.C., A. Grant and R.M. Faulks. 1975. Susceptibility of tubers to internal damage (blackspot). Potato Res 18:338–339.Google Scholar
  5. 5.
    Kaldy, M.S. and P. Markakis. 1972. Amino acid composition of selected potato varieties. Jour of Food Sci 37:375–377.CrossRefGoogle Scholar
  6. 6.
    Kapoor, A.C., S.L. Desborough, and P.H. Li 1975. Potato tuber proteins and their nutritional quality. Potato Res 18:469–478.CrossRefGoogle Scholar
  7. 7.
    Mathies, Gunter, and Hans-Dieter Belitz. 1978. Studies on enzymic browning of potatoes (Solanum tuberosum IV. Relationship between tyrosine turnover and rate of browning. Z Lebensom Unters Forsch 167:97–100.CrossRefGoogle Scholar
  8. 8.
    McDole, R.E., D.T. Westerman, G.D. Kleinschmidt, G.E. Kleinkopf and J. Ojala. 1987. Idaho fertilizer guide for potatoes. Current Information Series No. 261. University of Idaho, Moscow, Idaho.Google Scholar
  9. 9.
    Mondy, N.I., B.P. Klein and L.I. Smith. 1960. The effect of maturity and storage on phenolic content enzymatic activity and discoloration of potatoes. Food Res 25:693:705.Google Scholar
  10. 10.
    Olsson, K. and J.E. Fridell. 1975. Differences in blackspot susceptibility of two sib varieties of potato in relation to cell volume and some chemical factors. Abstract. Potato Res 18:338.Google Scholar
  11. 11.
    Pavek, J., D. Corsini and F. Nissley. 1985. A rapid method for determining blackspot susceptibility of potato clones. Am Potato J 62:511–517.Google Scholar
  12. 12.
    Pavek, J.J., C.R. Brown, M.W. Martin and D.L. Corsini. 1989. Inheritance of resistance to blackspot in potato. Abstract. Am Potato J 66:539.Google Scholar
  13. 13.
    Peterson, C.L. and C.W. Hall. 1975. Dynamic mechanical properties of the Russet Burbank potato as related to temperature and bruise susceptibility. Abstract. Am Potato J 52:289.CrossRefGoogle Scholar
  14. 14.
    Rathlef, H.V. 1932. Die stammtefeln des weltsortiments der kartoffel and ihre generativ fruchtbaren Sorten.In: Kuhn Archiv. Berlin, Arbeiten aus den Landwirtschaftlichen Instituten des Universitat Halle. Band 33:296-431. Paul Parey, Berlin, Pub.Google Scholar
  15. 15.
    Schaller, Klaus and Anton Amberger. 1974. Zusammenhange zwischen den fur die rohvefarbang der kartoffelknolle verantwortlishen inhaltsstoffen, Qual. Plant. — Pl Fds Hum Nut XXIV 1/2:183–190.CrossRefGoogle Scholar
  16. 16.
    Skrobacki, Alojzy, James L. Halderson, Joseph J. Pavek and Dennis Corsini. 1989. Determining potato tuber resistance to impact damage. Am Potato J 66:401–415.Google Scholar
  17. 17.
    Stark, J.C., D.L. Corsini, P.J. Hurley and R.B. Dwelle. 1985. Biochemical characteristics of potato clones differing in blackspot susceptibility. Am Potato J 62:657–666.Google Scholar
  18. 18.
    Undenfriend S. and L. Cooper. 1952. The chemical estimation of tyrosine and tyramine. J Biol Chem 196:227–233.Google Scholar
  19. 19.
    Vertregt, N. 1968. Relationship between blackspot and composition of the potato tuber. European Pot J 11:34–44.CrossRefGoogle Scholar
  20. 20.
    Woodwards, Lynne and M.T. Jackson. 1985. The lack of enzymic browning in wild potato species series Longipedicellota and their crossability withSolanum tuberosum. Z Pflanzenzuchtg 94:278–287.Google Scholar

Copyright information

© Springer 1992

Authors and Affiliations

  • Dennis L. Corsini
    • 1
  • Joseph J. Pavek
    • 1
  • Bill Dean
    • 2
  1. 1.USDA-ARSAberdeen
  2. 2.Washington State UniversityProsser, Washington

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