American Potato Journal

, Volume 74, Issue 1, pp 1–13 | Cite as

Comparison of polyphenol oxidase in tubers ofSolanum tuberosum and the non-browning tubers ofS. hjertingii

  • Sopheak K. Sim
  • Sarah M. Ohmann
  • Cindy B. S. Tong
Article
Accepted:

Abstract

Solarium hjertingii, a wild potato species, does not exhibit enzymatic browning nor blackspot. Blackspot and enzymatic browning are physiological disorders that are caused by the reaction of the enzyme polyphenol oxidase with phenolic substrates, resulting in discoloration of tuber cortex. We determined thatS. hjertingii has a low amount of at least one of the polyphenol oxidases in its tubers compared toS. tuberosum. Less than 5 μg ofS. tuberosum polyphenol oxidase protein, as determined from Western blot staining, had measurable enzymatic activity. In contrast,S. hjertingii protein with comparable staining had no detectable activity.

Additional Key Words

Blackspot chlorogenic acid enzymatic browning phenolics 

Compendio

Solarium hjertingii, una especie silvestre de papa, no presenta el bronceado enzimático ni la mancha negra. La mancha negra y el broncea.do enzimático son desórdenes fisiológicos causados por la reacción de la enzima polifenol oxidasa con los sustratos fenólicos, que dan lugar a la descoloración de la corteza del tubérculo. Nosotros determinamos queS. hjertingii tiene una baja concentración de por lo menos una de las fenol oxidasas en sus tubérculos, en comparación conS. tuberosum. Una cantidad menor de 5μg de la proteína polifenol oxidasa deS. tuberosum, determinada por tinción en Western blot, mostró una actividad enzimática que se podía medir. En contraste, la proteína deS. hjertingii con una tinción semejante no mostró actividad detectable.

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Copyright information

© Springer 1997

Authors and Affiliations

  • Sopheak K. Sim
  • Sarah M. Ohmann
  • Cindy B. S. Tong
    • 1
  1. 1.Department of Horticultural ScienceUniversity of MinnesotaSt. Paul

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