Expression of TGF-β2 in LECs of age-related nuclear, cortex cataract and the relationship among TGF-β2, proliferation, apoptosis and transdifferentiation

  • Ye Lin
  • Cai Xiaojun
  • Zhang Baifang
  • Luo Hong
  • Deng Ping


To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β2 mRNA, proliferation cell nuclear antigen (PCNA), Bcl-2/Bax, fibronection (FN), vimentin protein and the density of lens epithelial cells(LECs) of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β2, mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was (4250.63±275.05)/mm2 in cortex cataract. It was (5438.40±262.30)/mm2 in nuclear cataract, and (5368.63±211.07)/mm2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract (p<0.05). These suggested that TGF-β2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.

Key words

TGF-β2 PCNA Bcl-2/Bax FN vimentin cataract cortex nuclear 

CLC number

R 767 


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  1. [1]
    Li W C, Kuszak J R, Dunn K,et al. Lens Epithelial Cell Apoptosis Appears To Be a Common Cellular Basis for Non-congential Cataract Development in Human and Animals [J].J Cell Biol, 1995,130:169–181.CrossRefGoogle Scholar
  2. [2]
    Joo C K, Lee E H, Kim J C,et al. Degeneration and Transdifferentiation of Human Lens Epithelial Cells in Nuclear and Anterior Polar Cataract [J].Cataract Refract Surg, 1999,25(25):652–658.CrossRefGoogle Scholar
  3. [3]
    Sun Zhaohui. The Molecular Cell and Biologic Mechanism of Posterior Capsular Turbidity [J].Foreign Medical Science, 2001,24(3):150–153 (Ch).Google Scholar
  4. [4]
    Huang Y, Wei H. Expression of Transforming Growth Factor-beta in Cultured Normal Human Lens Epithlia Cells [J].J Huazhong Univ Sci Technolog Med Sci, 2004,24(3): 289–291.MathSciNetCrossRefGoogle Scholar
  5. [5]
    Wormstone I M, Tamiya S, Eldred J A. Characterisation of TGF-β2 Signalling and Function In Human Lens Cell Line [J].Exp Eye Res, 2004,78(3):705–714.CrossRefGoogle Scholar
  6. [6]
    Zhang Xiaorong, Li Xiaohong, Sun Huimin. Transforming Growth Factor-β Induce Apoptosis in Lens Epithelial Cells [J].Chin Ophthal Res, 2000,18:114–116 (Ch).Google Scholar
  7. [7]
    Hall A B, Thompson J R, Deane J S,et al. LocsIII Versus the Oxford Clinical Cataract Classification and Grading System for the Assessment of Nuclear, Cortical and Posterior Sub-Capsular Cataract [J].Ophthalmic Epidemiol, 1997,4 (4):179.CrossRefGoogle Scholar
  8. [8]
    Ginham R, Harrison D C, Facci L,et al. Upregulation of Death Pathway Molecules in Rat Care Bellar Granule Neurons Undergoing Apoptosis [J].Neurosci Lett, 2001,302(2–3): 113–116.CrossRefGoogle Scholar
  9. [9]
    Sun M A. Lens Epithelial Cell Differentiation [J].Exp Eye Res, 2002,75(5):485–490.CrossRefGoogle Scholar
  10. [10]
    Li W C, Spector A. Lens Epithelial Cell Apoptosis Is an Early Event in the Development of UVB-induced Cataract [J].Free Radic Biol Med, 1996,20:301–311.CrossRefGoogle Scholar
  11. [11]
    Kato K, Kurosaka D, Nagamoto T. Apoptotic Cell Death in Rabbit Lens after Extraction [J].Invest Ophthalmol Vis Sci, 1997,38:2322–2330.Google Scholar
  12. [12]
    Adama J M, Cory S. The Bcl-2 Protein Family: Arbiters of Cell Survival [J].Science, 1998,281:1322–1326.CrossRefGoogle Scholar
  13. [13]
    Zhang X H, Sun H M, Yuan J O. Extracellular Matrix Preduction of Lens Epithelial Cells [J].J Cataract Refract Surg, 2001,27(8):1303–1309.CrossRefGoogle Scholar
  14. [14]
    Thanamake T, Pelliniemi L J, Vuorio E. Collagens and Collage-Related Matrix Components in the Human and Mouse Eye [J].Pro Retin Eye Res, 2004,23(4):403–434.CrossRefGoogle Scholar
  15. [15]
    Park J C, Park B J, Suh H,et al. Comparative Study On Motility of the Cultured Fetal and Neonatal Dermal Fibroblasts-in Extracellular Matrix [J].Yonsei Med J. 2001,42 (6):587–594.Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Ye Lin
    • 1
  • Cai Xiaojun
    • 1
  • Zhang Baifang
    • 2
  • Luo Hong
    • 1
  • Deng Ping
    • 1
  1. 1.Ophthalmology Department, Zhongnan HospitalWuhan UniversityWuhan, HubeiChina
  2. 2.Medical SchoolWuhan UniversityWuhan, HubeiChina

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