Expression of TGF-β2 in LECs of age-related nuclear, cortex cataract and the relationship among TGF-β2, proliferation, apoptosis and transdifferentiation

  • Ye Lin
  • Cai Xiaojun
  • Zhang Baifang
  • Luo Hong
  • Deng Ping
Article
  • 37 Downloads

Abstract

To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β2 mRNA, proliferation cell nuclear antigen (PCNA), Bcl-2/Bax, fibronection (FN), vimentin protein and the density of lens epithelial cells(LECs) of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β2, mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was (4250.63±275.05)/mm2 in cortex cataract. It was (5438.40±262.30)/mm2 in nuclear cataract, and (5368.63±211.07)/mm2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract (p<0.05). These suggested that TGF-β2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.

Key words

TGF-β2 PCNA Bcl-2/Bax FN vimentin cataract cortex nuclear 

CLC number

R 767 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Ye Lin
    • 1
  • Cai Xiaojun
    • 1
  • Zhang Baifang
    • 2
  • Luo Hong
    • 1
  • Deng Ping
    • 1
  1. 1.Ophthalmology Department, Zhongnan HospitalWuhan UniversityWuhan, HubeiChina
  2. 2.Medical SchoolWuhan UniversityWuhan, HubeiChina

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