In Vitro

, Volume 12, Issue 11, pp 734–742 | Cite as

Study of markers of human erythroid differentiation in hybrid cells

  • Albert Deisseroth
  • Arthur Nienhuis
Symposium Procedings

Summary

Somatic cell hybrids exhibiting co-expression of the globin genes of two species were generated by fusion of mouse erythroleukemia cells with Chinese hamster or human marrow erythroid cells. In contrast, extinction of the mouse globin genes occurred in hybrids formed between the erythroleukemia cells and human fibroblasts. Direct detection of the human globin genes in human X mouse fibroblast hybrids was achieved by annealing of DNA from these cells to human globin complementary DNA. This method was developed to permit the chromosomal assignment of the human globin genes.

Key words

globin gene chromosomal localization regulation gene expression 
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Copyright information

© Tissue Culture Association 1977

Authors and Affiliations

  • Albert Deisseroth
    • 1
  • Arthur Nienhuis
    • 2
  1. 1.Children’s Hospital Medical CenterBoston
  2. 2.the Molecular Hematology Branch, National Heart, Lung and Blood InstituteNational Institutes of HealthBethesda

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