In Vitro

, Volume 12, Issue 1, pp 19–22 | Cite as

Effect of room fluorescent light on the deterioration of tissue culture medium

  • Richard J. Wang


A major cause of tissue culture medium deterioration is exposure to room fluorescent light. Riboflavin and tryptophan present in Dulbecco's modified Eagle's minimum essential medium, when exposed to light, yield toxic photoproducts responsible for loss of the ability of the medium to support clonal growth of human, mouse and Chinese hamster cell lines. Procedures for minimizing medium deterioration are discussed.

Key words

fluorescent light photoproduct media media deterioration clonal growth 


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Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • Richard J. Wang
    • 1
  1. 1.Division of Biological Sciences and Dalton Research CenterUniversity of Missouri-ColumbiaColumbia

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