Wuhan University Journal of Natural Sciences

, Volume 4, Issue 4, pp 495–497 | Cite as

Alcohol dehydrogenase activity in cultured cells from different tobacco (Nicotiana tabacum L.) varieties

  • Zheng Ling
  • Yang Yue-sheng
  • zheng Ying-dong
Article
Received:
  • 16 Downloads

Abstract

The activity of alcohol dehydrogenase (ADH) in cultured cells of various tobacco was determined. It was found that significant differences existed in cells of different varieties cultured under normal conditions and as well after treated with exogenous ethanol. The ADH activity had positive relation with the ability of the cells to catabolize exogenous ethanol, indicating that the main function of the ADH in tobacco cells was in the direction of converting ethanol to acetaldehyde.

Key words

alcohol dehydrogenase suspension-cultured cells tobacco 

CLC number

Q 554. 9 

Documrent code

A 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Davies D D. Anaerobic metabolism and the production of organic acids[A]. In:The Biochemistry of plant [C]. P K Stumph E E Conn, eds. New York: Academic Press, 1980,2: 581–611.Google Scholar
  2. [2]
    Roberts J K M, Callis J, Wemmer D,et al. Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia[J].Proc Natl Acad Sci USA, 1984a,81:3379–3383.CrossRefGoogle Scholar
  3. [3]
    Roberts J K M, Callis J, Wemmer D,et al. Cytoplasmic acidosis as a determinant of flooding intolerance in plants[J].Proc Natl Acad Sci USA, 1984b,81: 6029–6033.CrossRefGoogle Scholar
  4. [4]
    Jackson M B, Herman B, Goodenough A. An examination of the importance of ethanol in causing injury to flooded plants[J].Plant Cell Environ. 1982,5: 163–172.Google Scholar
  5. [5]
    Crawford R M M, Zochowski Z M. Tolerance of anoxia and ethanol toxicity in chickpea seedlings (Cicer arietinum L( [J].J Exp Bot, 1984,35: 1472–1480.CrossRefGoogle Scholar
  6. [6]
    Monk L S, Fagerstedt K V, Crawford R M M. Suteroxide dismutase as an anaerobic polypetide. A key factor in recovery from oxygen deprivation inIris psudacorus?[J]Plant Physiol, 1987,85:1016–1020.Google Scholar
  7. [7]
    MacDonld R C, Kimmerer T W. Metabolism of transpired ethanol by eastern cottonwood (Populus deltoides Bartr)[J].Plant Physiol, 1993,102:173–179.Google Scholar
  8. [8]
    YANG Y, JIAN Y: The role of ethanol metabolism in plants.J South China Univ Techn (Natural Sci), 1996,24 (suppl.): 98–102 (Ch).Google Scholar
  9. [9]
    Perata P, Alpi A. Ethanol metabolism in suspension cultured carrot cells[J].Physiol plant, 1991,82: 103–108.CrossRefGoogle Scholar
  10. [10]
    Yang Y, Chu D J, aizawa M,et al. Ethanol metabolism by tobacco callusin vitro[J].Plant Science, 1995,104: 147–151.CrossRefGoogle Scholar
  11. [11]
    Ikuo I, Masahide Y. Alcohol dehydrogenase from coultured rice cells[J].Plant & cell physiol. 1982,23 (2):213–225.Google Scholar
  12. [12]
    Roberts J K M, Chang K, Webseer C,et al. Dependence of ethanolic fermentation, cytoplasmic ph regulation and viability on the activity on alcohol dehydrogenase in hypoxic maize root tips[J].Plant Physiol, 1989,89:1275–1278.CrossRefGoogle Scholar
  13. [13]
    ZHENG Yin-dong. Regulation on the Ethanol Metabolism of Tobacco Tissuesin vitro [D]. Guongzhou: College of Biotechnology. Sowth china Agricultural University. 1998.Google Scholar

Copyright information

© Springer 1999

Authors and Affiliations

  • Zheng Ling
    • 1
  • Yang Yue-sheng
    • 1
  • zheng Ying-dong
    • 1
  1. 1.College of BiotechnologySouth China Agricultural UniversityGuanzhouChina

Personalised recommendations