Effects of rHu-EPO on myocyte apoptosis and cardiac function following acute myocardial infarction in rats
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- Liang, Y., Xinling, D., Jiahong, X. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2005) 25: 55. doi:10.1007/BF02831387
The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infraction in rats were studied. Thirty-two rats wre divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i. p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+EPO group was much better than in MI group. The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.
Key wordsrecombinant human erythropoietin myocardial infarction apoptosis
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