Recovery of DNA from agarose gel with home-made silica milk
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Abstract
An usefulness of silica milk made with waste ultraviolet light tube for recovery of DNA fragment from agarose gel was represented. The glass milk is a water suspension of 50% fine silica powder prepared by grinding the crushed waste ultraviolet light tube with a porcelain mortar. It was showed that one microliter of the glass milk could bind more than 1 μg of DNA fragment, and DNA fragment in length from 125 bp to 23 kb could be efficiently recovered from agarose gel. The bound DNA could be eluted from the particle of SiO2 in the glass milk with a yield of about 60%–80%. The eluted DNA could be used in all manipulations in molecular cloning.
Key words
silica milk DNA recovery usage of waste UV tubeCLC number
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References
- [1]Vogelstein B, Gillespie D, Preparative and Analytical Purification of DNA from Agarose.Proc Natl Acad Sci USA, 1979,76:615–619.CrossRefGoogle Scholar
- [2]Hengen P N, Methods and Reagents-Recovering DNA from Agarose Gels.Trends Biochem Sci, 1994,19:388–389.CrossRefGoogle Scholar
- [3]Heyd M L, Diehl J D Jr. Recovering DNA from Agarose Gels with Pumice.Bio Techniques, 1996,20: 394–398.Google Scholar
- [4]Carter M J, Miltor I D, An Inexpensive and Simple Method for DNA Purifications on Silica Particles.Nucleic Acid Res, 1993,21: 1044.CrossRefGoogle Scholar
- [5]Marko M A, Chipperfield R, Birboim H C, A Procedure for the Large-scale Isolation of Highly Purified Plasmid DNA Using Alkaline Extraction and Binding to Glass Powder.Anal Biochem, 1982,121:382–387.CrossRefGoogle Scholar
- [6]Sparks R B, Elder J H, A Simple and Rapid Procedure for the Purification of Plasmid DNA Using Reverse-phase C18 Silica Beads.Anal Biochem, 1983,135:345–348.CrossRefGoogle Scholar
- [7]Sambrook J, Fritsch E F, Manitiatis T,Molecular cloning: a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1989. 304–313.Google Scholar
- [8]Sambrook J, Fritsch E F, Manitiatis T,Molecular cloning: a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1989. 49–57.Google Scholar
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