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Plant Molecular Biology Reporter

, Volume 18, Issue 1, pp 5–16 | Cite as

Characterization of microsatellites and development of chromosome specific STMS markers in bread wheat

  • Rajeev K. Varshney
  • Alok Kumar
  • Harindra S. Balyan
  • Joy K. Roy
  • Manoj Prasad
  • Pushpendra K. Gupta
Commentary

Abstract

Microsatellites, or simple sequence repeats (SSRs), have become the markers of choice for genetic studies with many crop species including wheat. Currently an international effort is underway to enrich the repertoire of available sequence tagged microsatellite site (STMS) markers in wheat. As a part of this effort, we have sequenced 43 clones obtained from a microsatellite-enriched wheat genomic library; 34 clones contained 41 different microsatellites. These microsatellites (mono-, di-, tri- nucleotide repeats) were classified as 19 simple perfect, 18 simple imperfect and 4 compound imperfect types. Dinucleotide repeats were the most abundant (70%). Primer pairs for only 16 microsatellites could be designed, since the flanking sequences of the others were either too short or were otherwise not suitable for designing the microsatellite specific primers. Microsatellite loci of the expected size and polymorphism were successfully amplified from 15 of these 16 primer pairs using three wheat varieties. 14 loci detected by 12 out of the 15 functional primer pairs were assigned to 11 specific chromosomes.

Key words

Bread wheat chromosome assignment microsatellite molecular marker polymorphism 

Abbreviations

STMS

sequence-tagged-microsatellite-site

WMC

Wheat Microsatellite Consortium

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Copyright information

© Springer 2000

Authors and Affiliations

  • Rajeev K. Varshney
    • 1
  • Alok Kumar
    • 1
  • Harindra S. Balyan
    • 1
  • Joy K. Roy
    • 1
  • Manoj Prasad
    • 1
  • Pushpendra K. Gupta
    • 1
  1. 1.Molecular Biology Laboratory, Department of Agricultural BotanyCh. Charan Singh UniversityMeerutIndia

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