Plant Molecular Biology Reporter

, Volume 18, Issue 2, pp 133–138 | Cite as

Accessing genes in the tertiary gene pool of rice by direct introduction of total DNA fromZizania palustris (wild rice)

  • M. Abedinia
  • R. J. Henry
  • A. B. Blakeney
  • L. G. Lewin
Protocols

Abstract

Transfer of useful genes from wild relatives of crop plants has relied upon successful conventional crossing or the availability of the cloned gene. Co-bombardment of rice callus with total genomic DNA from wild rice (Zizania palustris) and a plasmid containing a gene confirming hygromycin resistance allowed recovery under selection of transgenic plants with grain characteristics from wild rice. Amplified Fragment Length Polymorphism (AFLP) analysis suggested that a significant amount of DNA fromZizania was introduced by this procedure. One plant had 16 of a possible 122Zizania specific AFLP markers detected with the primers used. This approach may have potential for introgression of genes from wild relatives in other cases where highly efficient transformation methods are available.

Key words

Oryza sativa rice wild rice whole genome transformation Zizania palustris 

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Copyright information

© International Society for Plant Molecular Biology 2000

Authors and Affiliations

  • M. Abedinia
    • 1
  • R. J. Henry
    • 1
  • A. B. Blakeney
    • 2
  • L. G. Lewin
    • 3
  1. 1.Centre for Plant Conservation GeneticsSouthern Cross UniversityLismoreAustralia
  2. 2.BRI AustraliaNorth RydeAustralia
  3. 3.Cooperative Research Centre for Sustainable Rice ProductionYanco Agricultural InstituteYancoAustralia

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