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Plant Molecular Biology Reporter

, Volume 18, Issue 2, pp 109–115 | Cite as

A simple procedure for the isolation of high quality RNA from ripening banana fruit

  • Mehar H. Asif
  • Puneet Dhawan
  • Pravendra Nath
Protocols

Abstract

Isolation of high quality RNA from ripening banana fruit tissue is difficult due to high levels of polysaccharides and other substances that interfere when using conventional procedures for RNA isolation. These substances not only decrease the yield but the quality of RNA is almost unusable. We describe here a simple RNA procedure that effectively removes these contaminating substances without affecting the yield. Following this procedure, we routinely obtained 80–150 μg of total RNA per g fresh tissue. The RNA is of good quality and suitable for northern analysis, RT-PCR and cDNA library construction.

Key words

Banana fruit tissue Musa acuminata polysaccharides RNA extraction 

Abbreviations

AMVRT

Avian Myelosblastosis Virus Reverse Transcriptase

CIP

Calf Intestine Alkaline Phosphatase

CTAB

cetyltrimethylammoniumbromide

DEAE

diethylaminoethyl

DEPC

diethyl pyrocarbonate

EtBr

ethidium bromide

EtOH

ethanol

RT-PCR

reverse transcription polymerase chain reaction

SDS

sodium dodecyl sulphate

SSC

saline sodium citrate

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Copyright information

© International Society for Plant Molecular Biology 2000

Authors and Affiliations

  1. 1.Plant Gene Expression LaboratoryNational Botanical Research InstituteLucknowIndia

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