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Micropropagation of 21 species of Mexican cacti by axillary proliferation

  • Eugenio Pérez Molphe Balch
  • Martha E. Pérez Reyes
  • Enrique Villalobos Amador
  • Ernestina Meza Rangel
  • Leticia del Rocío Morones Ruiz
  • Hugo J. Lizalde Viramontes
Micropropagation

Summary

We have developed micropropagation systems for 21 species of Mexican cacti using explants from seedlings germinatedin vitro or shoot segments of juvenile 2–3-yr-old greenhouse plants. The species propagated belong to the generaAstrophytum, Cephalocereus, Coryphantha, Echinocactus, Echinocereus, Echinofossulocactus, Ferocactus, Mammillaria, Nyctocereus, andStenocactus. Multiple shoot formation from areoles was achieved in Murashige and Skoog (MS) medium supplemented with either 1 or 2 mg N6-benzyladenine (BA) per 1 (4.44 or 8.87 μM) or BA at 1 or 2 mg/l plus naphthaleneacetic acid at 0.1 or 1 mg/l (0.54 or 5.37 μM). The requirements of growth regulators for optimal shoot proliferation, the velocity of the response, and the number of buds produced by explant were different among the genera and species studied. Rooting of the shoots generatedin vitro was achieved in MS medium supplemented with indoleacetic acid at 0.5–1 mg/l (2.85–5.71 μM) or indolebutyric acid at 0.5–1 mg/l (2.46–4.90 μM). Finally, 70–95% of the rooted plants transferred to potting medium survived.

Key words

Cactaceae tissue culture areole activation plant growth regulators plantlet regeneration 

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Copyright information

© Society for In Vitro Biology 1998

Authors and Affiliations

  • Eugenio Pérez Molphe Balch
    • 1
  • Martha E. Pérez Reyes
    • 1
  • Enrique Villalobos Amador
    • 1
  • Ernestina Meza Rangel
    • 1
  • Leticia del Rocío Morones Ruiz
    • 1
  • Hugo J. Lizalde Viramontes
    • 1
  1. 1.Dpto. de Química, Centro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientesMéxico

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