Protein secretion by gram-negative bacterial ABC exporters
- 107 Downloads
One of the strategies used by Gram-negative bacteria to secrete proteins across the two membranes which delimit the cells, issec independent and dedicated to proteins lacking an N-terminal signal peptide. It depends on ABC protein-mediated exporters, which consist of three cell envelope proteins: two inner membrane proteins: an ATPase (the ABC protein), a membrane fusion protein (MFP) and an outer membrane polypeptide.Erwinia chrysanthemi metalloproteinases B and C, andSerratia marcescens hemoprotein HasA are secreted by such homologous pathways and interact with the ABC protein. Interaction between the ABC protein and its substrate has also been evidenced by studies on proteinase and HasA hybrid transporters obtained by combining components from each system. Association between hemoprotein HasA and the three exporter/secretion proteins was demonstrated by affinity chromatography on hemin agarose on which the substrate remained bound with the three secretion proteins. The three component association was ordered and substrate binding was required for the formation of this multiprotein complex.
Unable to display preview. Download preview PDF.
- Akatsura H., Kawai E., Omori K., Shibatani T.: The three geneslipB, lipC, lipD involved in the extracellular secretion of theSerratia marcescens lipase which lacks an N-terminal signal peptide.J. Bacteriol. 177, 6381–6389 (1995).Google Scholar
- Boos W., Lucht J.M.: Periplasmic binding protein-dependent ABC transporters, pp. 1175–1209 in F.C. Neidhartet al., (Eds):Escherichia coli and Salmonella, Cellular and Molecular Biology. Amer. Soc. Microbiol. Press, Washington (DC) 1996.Google Scholar
- Delepelaire P., Wandersman C.: Protein secretion in Gram-negative bacteria. The extracellular metalloprotease B fromErwinia chrysanthemi, contains a C-terminal secretion signal analogous to that ofEscherichia coli α-haemolysin.J. Biol. Chem. 265, 9083–9089 (1990).Google Scholar
- Létoffé S., Delepelaire P. Wandersman C.: Protein secretion in Gram-negative bacteria: Assembly of the three components of ABC protein-mediated exporters is ordered and promoted by substrate binding.EMBO J., in press (1996).Google Scholar
- Mackman N., Nicaud J.M., Gray V., Holland I.B.: Secretion of hemolysin byEscherichia coli, pp. 159–181 inCurr. Topics Microbiol. Immunol, Vol. 125. Springer-Verlag, Berlin-Heidelberg 1986.Google Scholar
- Murphy C.K., Beckwith J.: Export of proteins to the cell envelope inEscherichia coli, pp. 967–978 in F.C. Neidhartet al. (Eds):Escherichia coli and Salmonella, Cellular and Molecular Biology. Amer. Soc. Microbiol. Press, Washington (DC) 1996.Google Scholar
- Wandersman C.: Secretion across the bacterial outer membrane, pp. 955–967 in F.C. Neidhartet al. (Eds):Escherichia coli and Salmonella typhimurium, Cellular and Molecular Biology. Amer. Soc. Microbiol. Press, Washington (DC) 1996.Google Scholar
- Welch R.A.: Pore-forming cytolysins of Gram-negative bacteria.Mol. Microbiol. 60, 101–124 (1991).Google Scholar
- Wolff N., Ghigo J.M., Delepelaire P., Wandersman C., Delepierre M.: C-Terminal secretion signal of anErwinia chrysanthemi protease secreted by a signal peptide-independent pathway: proton NMR and CD conformational studies in membranemimetic environments.Biochemistry 33, 6792–6801 (1994).PubMedCrossRefGoogle Scholar