Folia Microbiologica

, Volume 47, Issue 4, pp 458–460 | Cite as

Influence of calcium hydroxide root-canal sealer on microbial growthin vitro

  • S. Pezelj-Ribarić
  • I. Brekalo
  • M. Abram
  • M. Dorić
  • I. Miletić
  • Z. Karlović
Papers

Abstract

The calcium hydroxide-based filling material Apexit, which is often used in endodontic practice, was evaluated for its antibacterial and antifungal effects against microorganisms isolated from oral cavity (Serratia marcescens, Pseudomonas putida, Staphylococcus aureus, Candida albicans). Two different quantitative techniques were employed—the direct-contact test was used to examine the efficacy of freshly mixed material while the broth-survival test was employed to check the antimicrobial properties of 5-d-old material. Apexit inhibited Gram-negative bacteria more effectively than Gram-positive ones but had none or a very weak inhibitory effect onC. albicans.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdulkader A., Duguid R., Saunders E.M.: The antimicrobial activity of endodontic sealers to anaerobic bacteria.Internat. Endod. J. 29, 280–283 (1996).CrossRefGoogle Scholar
  2. Cannon R.D., Chaffin W.L.: Oral colonization byCandida albicans.Crit. Rev. Oral Biol. Med. 10, 359–383 (1999).PubMedCrossRefGoogle Scholar
  3. da Silva L.A., Leonardo M.R., Da Silva R.S., Assed S., Guimaräes L.F.L.: Calcium hydroxide root canal sealers: evaluation of pH, calcium ion concentration and conductivity.Internat. Endod. J. 30, 205–209 (1997).CrossRefGoogle Scholar
  4. Dorko E., Jenča A., Pilipčinec E., Danko J., Švický E., Tkáčiková L'.:Candida-associated denture stomatitis.Folia Microbiol. 46, 443–446 (2001a).CrossRefGoogle Scholar
  5. Dorko E., Zibrín M., Jenča A., Pilipčinec E., Danko J., Tkáčiková L’.: The histopathological characterization of oralCandida leukoplakias.Folia Microbiol. 46, 447–452 (2001b).CrossRefGoogle Scholar
  6. Estrela C., Pimenta F.C., Ito I.Y., Bammann L.L.:In vitro determination of direct antimicrobial effect of calcium hydroxide.J. Endod. 24, 15–17 (1998).PubMedCrossRefGoogle Scholar
  7. Fuss Z., Weiss E.I., Shalhav M.: Antibacterial activity of calcium hydroxide-containing endodontic sealers onEnterococcus faecalis in vitro.Internat. Endod J. 30, 397–402 (1997).CrossRefGoogle Scholar
  8. Heling I., Chandler N.P.: The animicrobial effect within dentinal tubules of four root canal sealers.J. Endod. 22, 257–259 (1996).PubMedCrossRefGoogle Scholar
  9. Kaplan A.E., Picca M., Gonzalez M.I., Macchi R.L., Molgatini S.L.: Antimicrobial effect of six endodontic sealers: anin vitro evaluation.Endod. Dent. Traumatol. 15, 42–45 (1999).PubMedCrossRefGoogle Scholar
  10. Mickel A.K., Wright E.R.: Growth inhibition ofStreptococcus anginosus (milleri) by three calcium hydroxide sealers and one zinc oxide-eugenol sealer.J. Endod. 25, 34–37 (1999).PubMedCrossRefGoogle Scholar
  11. Miletić I., Anić I., Pezelj-Ribarić S., Jukić S.: Leakage of five root canal sealers.Internat. Endod. J. 32, 415–418 (1999).CrossRefGoogle Scholar
  12. Miletić I., Anić I., Karlović Z., Maršan T., Pezelj-Ribarić S., Osmak M.: Cytotoxic effect of four root filling materials.Endod. Dent. Traumatol. 16, 287–290 (2000).PubMedCrossRefGoogle Scholar
  13. Peters L.B., Wesselink P.R., Moorer W.R.: The fate and the role of bacteria left in root canal tubules.Internat. Endod. J. 28, 95–99 (1995).CrossRefGoogle Scholar
  14. Siqueira J.F. Jr.,Lopes H.P.: Mechanisms of antimicrobial activity of calcium hydroxide: a critical review.Internat. Endod. J. 32, 361–369 (1999).CrossRefGoogle Scholar
  15. Waltimo T.M.T., Sirén E.K., Torkko H.L.K., Olsen I., Haapasalo M.P.P.: Fungi in therapy-resistant apical periodontitis.Internat. Endod. J. 30, 96–101 (1997).CrossRefGoogle Scholar
  16. Waltimo T.M.T., Sirén E.K., Orstavik D., Haapasalo M.P.P.: Susceptibility of oralCandida species to calcium hydroxidein vitro.Internat. Endod. J. 32, 94–98 (1999).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2002

Authors and Affiliations

  • S. Pezelj-Ribarić
    • 1
  • I. Brekalo
    • 1
  • M. Abram
    • 2
  • M. Dorić
    • 2
  • I. Miletić
    • 3
  • Z. Karlović
    • 3
  1. 1.Department of Oral Pathology, Department of Stomatology, Medical FacultyUniversity of RijekaCroatia
  2. 2.Department of Microbiology, Medical FacultyUniversity of RijekaCroatia
  3. 3.Department of Dental Pathology, Faculty of StomatologyUniversity of ZagrebCroatia

Personalised recommendations